SARS-CoV-2 detection using reverse transcription strand invasion based amplification and a portable compact size instrument
| Autor: | Frederikke Holm Jensen, Mirko Brummer, Julie Hindkær, Ebbe Finding, Peter Warthoe, Tuomas Ojalehto, Jakob Broberg Lind, Uffe Vest Schneider, Steven Clauwaert, Mette Bjergaard Dragheim, Julie Bjerring, Jan Gorm Lisby, Kristina Christensen, Antti Tullila, Helen Webb, Claus Juel Jensen, E. Thomas Danielsen, Charlotta Polacek, Mikkel Rohde Jacobsen, Maiken Worsøe Rosenstierne, Anders Fomsgaard, Robert Elkjær, Johnny Weber Lau, Shreya Joshi, Julie Blauenfeldt, Dac Mui Luong, Rikke Lind Jørgensen, Lars Bangsgaard, Lærke Jørgensen, Thomas Warthoe, Ask Bojesen, Flemming Holck |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Coronavirus disease 2019 (COVID-19)
Science Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Loop-mediated isothermal amplification Oropharynx Polymorphism Single Nucleotide Sensitivity and Specificity Article Humans Viral rna Strand invasion Pandemics Retrospective Studies Multidisciplinary Chemistry SARS-CoV-2 Reverse Transcriptase Polymerase Chain Reaction fungi COVID-19 Integrated optics Equipment Design Virology Mobile Applications Reverse transcriptase Molecular Diagnostic Techniques Viral infection Point-of-Care Testing Nucleic acid Medicine RNA Viral Cell Phone |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Rapid nucleic-acid based tests that can be performed by non-professionals outside laboratory settings could help the containment of the pandemic SARS-CoV-2 virus and may potentially prevent further widespread lockdowns. Here, we present a novel compact portable detection instrument (the Egoo Health System) for extraction-free detection of SARS-CoV-2 using isothermal Reverse Transcription Strand Invasion Based Amplification (RT-SIBA). The SARS-CoV-2 RT-SIBA assay can be performed directly on crude oropharyngeal swabs without nucleic acid extraction with a reaction time of 30 minutes. The Egoo health system uses a capsule system, which is automatically sealed tight in the Egoo instrument after applying the sample, resulting in a closed system optimal for molecular isothermal amplification. The performance of the Egoo Health System is comparable to the PCR instrument with an analytical sensitivity of 25 viral RNA copies per SARS-CoV-2 RT-SIBA reaction and a clinical sensitivity and specificity between 87.0-98.4% and 96.6-98.2% respectively. |
| Databáze: | OpenAIRE |
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