Tumor Necrosis Factor-α Induces p53-dependent Apoptosis in Rat Glioma Cells
| Autor: | Gene H. Barnett, Tatsuo Morimura, Juji Takeuchi, Seiji Kondo, Dali Yin |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Programmed cell death
medicine.medical_treatment DNA Mutational Analysis Apoptosis Biology Cycloheximide Cell Line chemistry.chemical_compound Glioma Gene expression Tumor Cells Cultured medicine Animals Dose-Response Relationship Drug Brain Neoplasms Tumor Necrosis Factor-alpha Brain medicine.disease Recombinant Proteins In vitro Rats Gene Expression Regulation Neoplastic Cytokine Animals Newborn chemistry Cancer research Surgery Tumor necrosis factor alpha Neurology (clinical) Tumor Suppressor Protein p53 |
| Zdroj: | Neurosurgery. 37:758-763 |
| ISSN: | 1524-4040 0148-396X |
| DOI: | 10.1227/00006123-199510000-00021 |
| Popis: | In this study, we demonstrated that tumor necrosis factor (TNF)-alpha inhibited the viability of rat glioma (C6) cells and induced apoptosis but did not affect the viability of rat newborn brain, mainly astroglial cells. The antitumor activity of TNF-alpha against C6 cells was partially inhibited by actinomycin D and cycloheximide, suggesting that it is possibly dependent upon new ribonucleic acid and protein synthesis. The results of immunoblotting assay demonstrated that TNF-alpha decreased the expression of mutant p53 protein but induced the expression of wild-type p53 in C6 cells during apoptosis. We suggest that TNF-alpha may activate the function of wild-type p53 protein by the suppression of mutant p53, at least indirectly, and induce p53-dependent apoptosis in glioma cells. |
| Databáze: | OpenAIRE |
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