Impaired LEF1 Activation Accelerates iPSC-Derived Keratinocytes Differentiation in Hutchinson-Gilford Progeria Syndrome
| Autor: | Xiaojing Mao, Zheng-Mei Xiong, Huijing Xue, Markus A. Brown, Yantenew G. Gete, Reynold Yu, Linlin Sun, Kan Cao |
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| Rok vydání: | 2022 |
| Předmět: |
Keratinocytes
congenital hereditary and neonatal diseases and abnormalities integumentary system Lymphoid Enhancer-Binding Factor 1 HGPS progeria lamin A WNT signaling LEF1 skins epidermal development keratins Organic Chemistry Induced Pluripotent Stem Cells nutritional and metabolic diseases Cell Differentiation General Medicine Lamin Type A Catalysis Computer Science Applications Inorganic Chemistry Progeria Humans Physical and Theoretical Chemistry Molecular Biology Wnt Signaling Pathway Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 10; Pages: 5499 |
| ISSN: | 1422-0067 |
| Popis: | Hutchinson-Gilford progeria syndrome (HGPS) is a detrimental premature aging disease caused by a point mutation in the human LMNA gene. This mutation results in the abnormal accumulation of a truncated pre-lamin A protein called progerin. Among the drastically accelerated signs of aging in HGPS patients, severe skin phenotypes such as alopecia and sclerotic skins always develop with the disease progression. Here, we study the HGPS molecular mechanisms focusing on early skin development by differentiating patient-derived induced pluripotent stem cells (iPSCs) to a keratinocyte lineage. Interestingly, HGPS iPSCs showed an accelerated commitment to the keratinocyte lineage than the normal control. To study potential signaling pathways that accelerated skin development in HGPS, we investigated the WNT pathway components during HGPS iPSCs-keratinocytes induction. Surprisingly, despite the unaffected β-catenin activity, the expression of a critical WNT transcription factor LEF1 was diminished from an early stage in HGPS iPSCs-keratinocytes differentiation. Chromatin immunoprecipitation (ChIP) experiment further revealed strong bindings of LEF1 to the early-stage epithelial developmental markers K8 and K18 and that the LEF1 silencing by siRNA down-regulates the K8/K18 transcription. During the iPSCs-keratinocytes differentiation, correction of HGPS mutation by Adenine base editing (ABE), while in a partial level, rescued the phenotypes for accelerated keratinocyte lineage-commitment. ABE also reduced the cell death in HGPS iPSCs-derived keratinocytes. These findings brought new insight into the molecular basis and therapeutic application for the skin abnormalities in HGPS. |
| Databáze: | OpenAIRE |
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