Simultaneous multicompartment intracellular Ca2+ measurements in the perfused heart using 19F NMR spectroscopy
| Autor: | Cameron S. Luo, Gerald M. Pohost, James A. Balschi, Martin M. Pike, Shigeki Yanagida |
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| Rok vydání: | 1996 |
| Předmět: |
Magnetic Resonance Spectroscopy
Biological Transport Active Fluorine-19 NMR Nuclear magnetic resonance Cytosol Organelle Animals Radiology Nuclear Medicine and imaging Egtazic Acid Lagomorpha biology Chemistry Endoplasmic reticulum Myocardium Resonance Heart Nuclear magnetic resonance spectroscopy Fluorine biology.organism_classification Perfusion Sarcoplasmic Reticulum Calcium Indicators and Reagents Rabbits Intracellular |
| Zdroj: | Magnetic resonance in medicine. 35(5) |
| ISSN: | 0740-3194 |
| Popis: | Although Ca2+ transport regulation at subcellular organelles is of great interest, only limited methodology has been available for measuring organellar [Ca2+] levels. The present study employs the 19F NMR resonance frequency of 4F-BAPTA to measure free [Ca2+]. In 4F-BAPTA loaded perfused rabbit hearts, two 19F NMR resonances were clearly observed. The frequency of one was consistent with cytosolic [Ca2+] levels. Responses to agents that after sarcoplasmic reticulum function identified the other resonance as originating from that organelle. The experiments demonstrate the utility of NMR shift indicator methodology in obtaining simultaneous multi-compartment intracellular [Ca2+] measurements and in enabling organellar [Ca2+] measurements to be made from within intact living tissue. |
| Databáze: | OpenAIRE |
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