Morphokinetic Characteristics and Developmental Potential of In Vitro Cultured Embryos from Natural Cycles in Patients with Poor Ovarian Response
| Autor: | N. Hojnik, V Vlaisavljević, B Kovačič |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Anti-Mullerian Hormone 0301 basic medicine animal structures Pregnancy Rate Article Subject Uterus lcsh:Medicine Fertilization in Vitro Biology Cleavage (embryo) General Biochemistry Genetics and Molecular Biology Embryo Culture Techniques Andrology 03 medical and health sciences 0302 clinical medicine Ovulation Induction Pregnancy medicine Humans Embryo Implantation Sperm Injections Intracytoplasmic 030219 obstetrics & reproductive medicine Zygote General Immunology and Microbiology lcsh:R Embryo General Medicine Embryo Transfer medicine.disease Pregnancy rate Blastocyst 030104 developmental biology medicine.anatomical_structure embryonic structures Oocytes Female Cytokinesis Embryo quality Research Article |
| Zdroj: | BioMed Research International, Vol 2016 (2016) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2016/4286528 |
| Popis: | Background. Patients with poor ovarian response to ovarian hyperstimulation represent an interesting group for studying the impact of embryo cleavage irregularities on clinical outcome since all embryos, regardless of their quality, are usually transferred to the uterus. The aim of our study was to follow the morphokinetics of fertilized oocytes from natural cycles in poor responders. Methods. Zygotes from 53 cycles were cultured in vitro for 3 days. The morphokinetics of their development and transfer outcomes were retrospectively analyzed for the normally and irregularly cleaved embryos. Results. Of all embryos, 30.2% had single and 20.8% multiple cleavage irregularities with the following prevalence: developmental arrest 30.2%, direct cleavage to more than two cells 24.5%, chaotic cleavage 13.2%, and reverse cleavage 11.3%. These embryos had longer pronuclear phases, first cytokinesis, second embryo cell cycles, and less synchronized divisions. The transfer of normally developing embryos resulted in an implantation rate of 30.8% and a delivery rate of 23.1%, but irregularly cleaved embryos did not implant. Conclusions. The use of time-lapse microscopy in poor responder patients identified embryos with cleavage abnormalities that are related with no or extremely low implantation potential. Gained information about embryo quality is important for counselling patients about their expectations. |
| Databáze: | OpenAIRE |
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