Infrared Laser Ablation Microsampling with a Reflective Objective

Autor:	Chao Dong, Luke T. Richardson, Touradj Solouki, Kermit K. Murray
Rok vydání:	2022
Předmět:	Brain Chemistry Proteomics Tandem Mass Spectrometry Structural Biology Lasers Animals Proteins Laser Therapy Spectroscopy Chromatography Liquid Rats
Zdroj:	Journal of the American Society for Mass Spectrometry. 33:463-470
ISSN:	1879-1123 1044-0305
DOI:	10.1021/jasms.1c00306
Popis:	A Schwarzschild reflective objective with a numerical aperture of 0.3 and working distance of 10 cm was used for laser ablation sampling of tissue for off-line mass spectrometry. The objective focused the laser to a diameter of 5 μm and produced 10 μm ablation spots on thin ink films and tissue sections. Rat brain tissue sections 50 μm thick were ablated in transmission geometry, and the ablated material was captured in a microcentrifuge tube containing solvent. Proteins from ablated tissue sections were quantified with a Bradford assay, which indicated that approximately 300 ng of protein was captured from a 1 mm
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::837946d2d51e2d0f56f234221878298e https://doi.org/10.1021/jasms.1c00306 Zobrazit plný text záznamu