Intralipid adversely affects reticuloendothelial bacterial clearance
| Autor: | Schmuel Katz, Brian R. Plaisier, Jay L. Grosfeld, Walter J. Folkening |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Fat Emulsions Intravenous Parenteral Nutrition medicine.medical_specialty medicine.medical_treatment Bacteremia Spleen medicine.disease_cause Internal medicine medicine Animals Lung Mononuclear Phagocyte System Escherichia coli Saline Escherichia coli Infections Analysis of Variance Kidney biology business.industry Rats Inbred Strains General Medicine Mononuclear phagocyte system biology.organism_classification Rats Surgery medicine.anatomical_structure Endocrinology Parenteral nutrition Pediatrics Perinatology and Child Health business Bacteria |
| Zdroj: | Journal of Pediatric Surgery. 26:921-924 |
| ISSN: | 0022-3468 |
| DOI: | 10.1016/0022-3468(91)90837-j |
| Popis: | Lipid emulsion is a major caloric source in patients receiving total parenteral nutrition. Cleared by the Kuppfer cells of the reticuloendothelial system (RES), lipid emulsion may adversely affect the RES function by decreasing its ability to remove blood-borne bacteria. This study evaluates and compares the blood clearance and organ localization of viable radiolabeled [ 36 S] Escherichia coli following slow intraperitoneal (IP) and more rapid intravenous (IV) administration of a 20% fat emulsion (FE). Sixty male Sprague-Dawley rats weighing 150 g were placed in 6 experimental groups (10 rats per group). Group I received IP normal saline (3 mL/d for 3 days); group II received IP FE (20%) (4 g/kg/d for 3 days); and group III received normal saline IV (3 mL/d for 3 days). The remaining animals received a slow (15-minute) IV infusion of FE (4 g/kg/dose) prior to bacterial challenge: group IV at 4 hours; group V at 24 hours; and group IV 4 at and 24 hours. E coli (10 6 /mL) were injected via the tail vein. Blood samples were obtained for clearance study. At 10 minutes, tissue samples (50 to 100 mg) of liver, spleen, kidney, and lung were obtained and processed for liquid scintillation counting. Although rapid bacterial blood clearance was found in all the groups, there was a significant change in organ localization of bacteria. Normal distribution of bacteria in group I was as follows: liver 70.1% ± 6.2%, spleen 5.2% ± 1.2%, kidney 0.2% ± 0.04%, and lung 1.6% ± 0.6%. There was a slight increase in lung localization of bacteria in rats receiving IP FE (3.7% ± 1.5%; P E coli , and may result in increased susceptibility to infection. These observations suggest that FE emulsion should be cautiously administered in regard to dose and rate in septic patients. |
| Databáze: | OpenAIRE |
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