Detection of Actinobacillus pleuropneumoniae by PCR on field strains from healthy and diseased pigs
| Autor: | Irene Silveira Schrank, Sergio Ceroni da Silva, Catia Silene Klein, Itamar Antônio Piffer, Augusto Schrank, Maria Bernardete Burin Fávero |
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| Rok vydání: | 2003 |
| Předmět: |
Serotype
DNA Bacterial Swine animal diseases Population Biology Applied Microbiology and Biotechnology Microbiology Polymerase Chain Reaction Sensitivity and Specificity law.invention Actinobacillus Infections law medicine Animals Serotyping education Pleuropneumonia Contagious Actinobacillus pleuropneumoniae Polymerase chain reaction Antiserum Swine Diseases education.field_of_study Pasteurellaceae General Medicine Sequence Analysis DNA respiratory system bacterial infections and mycoses medicine.disease biology.organism_classification Virology respiratory tract diseases Blotting Southern Pleuropneumonia Bacteria |
| Zdroj: | Current microbiology. 46(6) |
| ISSN: | 0343-8651 |
| Popis: | We investigated whether primers able to specifically amplify a 0.7-kb DNA fragment from the conserved cpx genes could be applied to analyze A. pleuropneumoniae field isolates. The specific cpx primers were tested on 120 strains of A. pleuropneumoniae and other NAD-dependent field isolates from healthy and diseased animals to analyze A. pleuropneumoniae isolates from pigs in Brazil. We found that PCR and hybridization were able to discriminate between isolates of A. pleuropneumoniae and other bacteria. The 0.7-kb cpx DNA fragments were amplified from all 63 A. pleuropneumoniae isolates from herds with clinical symptoms and were isolated from lesions of acute cases of swine pleuropneumonia, both serotypable and nonserotypable. The PCR was also applied to 57 field isolates obtained from animals of apparently healthy herds, and the amplified cpx product was present in four serotypable and only two out of eleven A. pleuropneumoniae nonserotypable isolates. All nonserotypable A. pleuropneumoniae isolates revealed the apxA amplification pattern compatible with previously known serotypes. Some nonserotypable isolates might represent a population of isolates that originally were serotypable but lost the ability to react with serotype-specific antisera or might belong to novel serotypes. The PCR method applied is highly sensitive for serotypable A. pleuropneumoniae strains and for nonserotypable strains isolated from acute cases of swine pleuropneumoniae in Brazil. |
| Databáze: | OpenAIRE |
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