Hierarchical Macroporous Particles for Efficient Whole-Cell Immobilization: Application in Bioconversion of Greenhouse Gases to Methanol
| Autor: | Yale Jeon, Dong Rip Kim, Min Soo Jeon, Sun Chang Kim, Jung-Kul Lee, Byung-Kwan Cho, Vipin Chandra Kalia, Rahul K. Gupta, Sanjay K.S. Patel |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Materials science Bioconversion 01 natural sciences Methane 03 medical and health sciences chemistry.chemical_compound Greenhouse Gases Biogas 010608 biotechnology General Materials Science 030304 developmental biology 0303 health sciences Bacteria Methanol Cells Immobilized Carbon chemistry Chemical engineering Covalent bond Greenhouse gas Biofuels Carbon dioxide Leaching (metallurgy) Porosity Biotechnology |
| Zdroj: | ACS applied materialsinterfaces. 11(21) |
| ISSN: | 1944-8252 |
| Popis: | A viable approach for methanol production under ambient physiological conditions is to use greenhouse gases, methane (CH4) and carbon dioxide (CO2), as feed for immobilized methanotrophs. In the present study, unique macroporous carbon particles with pore sizes in the range of ∼1-6 μm were synthesized and used as support for the immobilization of Methylocella tundrae. Immobilization was accomplished covalently on hierarchical macroporous carbon particles. Maximal cell loading of covalently immobilized M. tundrae was 205 mgDCM g-1 of particles. Among these particles, the cells immobilized on 3.6 μm pore size particles showed the highest reusability with the least leaching and were chosen for further study. After immobilization, M. tundrae showed up to 2.4-fold higher methanol production stability at various pH and temperature values because of higher stability and metabolic activity than free cells. After eight cycles of reuse, the immobilized cells retained 18.1-fold higher relative production stability compared to free cells. Free and immobilized cells exhibited cumulative methanol production of 5.2 and 9.5 μmol mgDCM-1 under repeated batch conditions using simulated biogas [CH4 and CO2, 4:1 (v/v)] as feed, respectively. The appropriate pore size of macroporous particles favors the efficient M. tundrae immobilization to retain better biocatalytic properties. This is the first report concerning the covalent immobilization of methanotrophs on the newly synthesized macroporous carbon particles and its subsequent application in repeated methanol production using simulated biogas as a feed. |
| Databáze: | OpenAIRE |
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