Susceptibility of Phelipanche and Orobanche species to AAL-toxin
| Autor: | Axel de Zélicourt, Grégory Montiel, Philippe Delavault, Philippe Simier, Jean-Bernard Pouvreau, Séverine Thoiron, Sabine Delgrange |
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| Rok vydání: | 2009 |
| Předmět: |
Fusarium
Parasitic plant Molecular Sequence Data Germination DNA Fragmentation Plant Science Fungus Alternaria alternata Microbiology Orobanchaceae Species Specificity Sphingosine otorhinolaryngologic diseases Genetics Amino Acid Sequence Cloning Molecular Plant Diseases Plant Proteins Cell Death Sequence Homology Amino Acid biology Orobanche Fungi imperfecti biology.organism_classification Alternaria Seedlings Seeds Sequence Alignment |
| Zdroj: | Planta. 230:1047-1055 |
| ISSN: | 1432-2048 0032-0935 |
| DOI: | 10.1007/s00425-009-1008-1 |
| Popis: | Fusarium and Alternaria spp. are phytopathogenic fungi which are known to be virulent on broomrapes and to produce sphinganine-analog mycotoxins (SAMs). AAL-toxin is a SAM produced by Alternaria alternata which causes the inhibition of sphinganine N-acyltransferase, a key enzyme in sphingolipid biosynthesis, leading to accumulation of sphingoid bases. These long chain bases (LCBs) are determinant in the occurrence of programmed cell death (PCD) in susceptible plants. We showed that broomrapes are sensitive to AAL-toxin, which is not common plant behavior, and that AAL-toxin triggers cell death at the apex of the radicle as well as LCB accumulation and DNA laddering. We also demonstrated that three Lag1 homologs, encoding components of sphinganine N-acyltransferase in yeast, are present in the Orobanche cumana genome and two of them are mutated leading to an enhanced susceptibility to AAL-toxin. We therefore propose a model for the molecular mechanism governing broomrape susceptibility to the fungus Alternaria alternata. |
| Databáze: | OpenAIRE |
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