Human sperm proteins from testicular and epididymal origin that participate in fertilization: modulation of sperm binding to zona-free hamster oocytes, using monoclonal antibodies
| Autor: | Clotilde Duquenne, Bruno Lassalle, Jacques Testart, C. Finaz, Anick Lefevre, Svetlozara Villaroya, Franck Boué |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
endocrine system medicine.medical_specialty medicine.drug_class Hamster Testicle Biology In Vitro Techniques Monoclonal antibody Male infertility Andrology Mice Antigen Internal medicine Cricetinae Testis Genetics medicine Animals Humans Antigens reproductive and urinary physiology Epididymis Sperm-Ovum Interactions urogenital system Antibodies Monoclonal Membrane Proteins Cell Biology medicine.disease Sperm Immunohistochemistry Spermatozoa Sperm Maturation Endocrinology medicine.anatomical_structure Fertilization biology.protein Oocytes Female Antibody Developmental Biology |
| Zdroj: | Molecular reproduction and development. 33(4) |
| ISSN: | 1040-452X |
| Popis: | In order to identify human sperm surface proteins involved in the gamete recognition process, mouse monoclonal antibodies were directed against human spermatozoa and screened with live spermatozoa by enzyme-linked immunosorbent assay (ELISA). Immunoperoxydase staining of human testis showed the early presence of four corresponding proteins on germinal cells, while six were detected primarily in testis fluid. The presence of 17 proteins was evidenced in the epididymis. Eight were detected with a decreasing gradient from the begining to the end of the organ, including vasa efferentia for three of them. The other nine were observed in only one defined segment, usually the caput epididymis, which was found to be the most active region. Comparison of spermatozoa patterns from testis, vasa efferentia, and the three regions of epididymis pointed out a progressive coating. By contrast, three antibodies displayed a migration of spermatozoa surface domains in the course of epididymal transit. Six antibodies were found to inhibit human spermatozoa adherence to zona-free hamster oocytes, while nine promoted it. Molecular weights of antigens corresponding to nine of the antibodies ranged from 11 to 215 kDa. No correlation could be established with previously described human proteins. These observations emphasize the role of epididymis in human sperm maturation. © 1992 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text