βIII spectrin controls the planarity of Purkinje cell dendrites by modulating perpendicular axon-dendrite interactions
| Autor: | Mineko Kengaku, Junko Kurisu, Kazuto Fujishima, Midori Yamada |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Purkinje cell
Dendrite Cerebellar Purkinje cell Cell Communication Biology 03 medical and health sciences Mice Purkinje Cells 0302 clinical medicine Microtubule Cerebellum medicine Biological neural network Animals Humans Spinocerebellar Ataxias Spectrin Axon Cytoskeleton Molecular Biology Cells Cultured 030304 developmental biology 0303 health sciences Chemistry Dendrites Granule cell Axons medicine.anatomical_structure nervous system Biophysics 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Development (Cambridge, England). 147(24) |
| ISSN: | 1477-9129 |
| Popis: | The mechanism underlying the geometrical patterning of axon and dendrite wiring remains elusive, despite its critical importance in the formation of functional neural circuits. Cerebellar Purkinje cell (PC) arborizes a typical planar dendrite, which forms an orthogonal network with granule cell (GC) axons. By using electrospun nanofiber substrates, we reproduce the perpendicular contacts between PC dendrites and GC axons in culture. In the model system, PC dendrites show preference to grow perpendicular to aligned GC axons, which presumably contribute to the planar dendrite arborizationin vivo. We show that βIII spectrin, a causal gene for spinocerebellar ataxia type 5 (SCA5), is required for the biased growth of dendrites. βIII spectrin deficiency causes actin mislocalization and excessive microtubule invasion in dendritic protrusions, resulting in abnormally oriented branch formation. Furthermore, disease-associated mutations affect the ability of βIII spectrin to control dendrite orientation. These data indicate that βIII spectrin organizes the dendritic cytoskeleton and thereby regulates the oriented growth of dendrites with respect to the afferent axons.Summary statementβIII spectrin suppress the microtubule dynamics at the neuronal dendrite to inhibit the abnormal lateral branching, which causes misoriented branch formation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|