Cytosine–phosphate–guanosine-DNA induces CD274 expression in human B cells and suppresses T helper type 2 cytokine production in pollen antigen-stimulated CD4-positive cells
| Autor: | Youko Osawa, Norihiko Narita, Yumi Ito, Takechiyo Yamada, Seita Kubo, Shigeharu Fujieda |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
T cell
Programmed Cell Death 1 Receptor Immunology B-cell receptor Cell Communication Biology Lymphocyte Activation B7-H1 Antigen Inducible T-Cell Co-Stimulator Ligand Interferon-gamma Interleukin 21 Th2 Cells Adjuvants Immunologic medicine Humans Immunology and Allergy IL-2 receptor CXCL13 Cells Cultured Plant Proteins B-Lymphocytes Interleukin-13 CD40 NF-kappa B p50 Subunit Rhinitis Allergic Seasonal Original Articles Antigens Plant Interleukin-12 Interleukin 10 medicine.anatomical_structure Oligodeoxyribonucleotides biology.protein Interleukin 12 Cytokines Pollen Interleukin-5 |
| Zdroj: | Clinical and Experimental Immunology. 169:1-9 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1111/j.1365-2249.2012.04585.x |
| Popis: | Summary Co-stimulatory molecules are important for regulating T cell activation and immune response. CD274 [programmed death ligand 1 (PD-L1), B7-H1] has emerged as an important immune modulator that can block T cell receptor signalling. We have investigated whether PD-L1 and other co-stimulatory ligands could be expressed in human B cells stimulated by cytosine–phosphate–guanosine (CpG)-DNA. CpG-DNA strongly induced the co-inhibitory molecule ligand, PD-L1, of human B cells. Results show that nuclear factor-kappa B (NF-κB) signalling is involved directly in CpG-DNA-induced PD-L1 expression in human B cells. We sought to determine the effect of CpG-DNA-treated B cells on T helper type 2 (Th2) cytokine production in Cry j 1 (Japanese pollen antigen)-stimulated human CD4-positive cells from patients with seasonal allergic rhinitis caused by Japanese cedar pollen. CpG-DNA-treated B cells reduced Cry j 1-induced interleukin (IL)-5 and IL-13 production in CD4-positive cells. When the binding of PD-1 to PD-L1 was inhibited by PD-1-immunoglobulin (Ig), this chimera molecule reversed the previously described reductions in IL-5 and IL-13 production. In contrast, the CpG B-treated B cells increased both interferon (IFN)-γ and IL-12 production in the presence of Cry j 1-stimulated CD4-positive cells. CpG-DNA simultaneously reduced the expression of B7RP-1 [also known as inducible co-stimulator ligand (ICOSL), B7-H2] and the ligand of CD30 (CD30L). These results indicate that CpG-DNA induces co-inhibitory molecule ligand PD-L1 expression in human B cells and PD-L1 can suppress Th2 cytokine production in Cry j 1-stimulated CD4-positive cells, while CpG-DNA increased Th1 cytokine production and reduced the expression of co-stimulatory molecule ligands that can promote Th2 inflammatory responses. |
| Databáze: | OpenAIRE |
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