TNF Production from Peripheral Blood Mononuclear Cells in Diabetic Patients after Stimulation with Alginate and Lipopolysaccharide
| Autor: | Gudmund Skjåk-Bræk, I. Følling, Terje Espevik, Bård Kulseng |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Lipopolysaccharides Male medicine.medical_specialty Necrosis Calcium alginate Adolescent Lipopolysaccharide Alginates Immunology Biocompatible Materials Stimulation Peripheral blood mononuclear cell chemistry.chemical_compound Glucuronic Acid Diabetes mellitus Internal medicine medicine Humans Tumor Necrosis Factor-alpha business.industry Hexuronic Acids General Medicine Middle Aged medicine.disease Diabetes Mellitus Type 1 Uronic Acids medicine.anatomical_structure Endocrinology chemistry Leukocytes Mononuclear Female Tumor necrosis factor alpha medicine.symptom Pancreas business |
| Zdroj: | Scandinavian Journal of Immunology. 43:335-340 |
| ISSN: | 1365-3083 0300-9475 |
| DOI: | 10.1046/j.1365-3083.1996.d01-43.x |
| Popis: | Microencapsulated pancreatic Langerhan's islets in calcium alginate gels have been used as an implantable bio-artificial pancreas in the treatment of diabetes mellitus, but with limited success due to overgrowth of the capsule with fibroblasts and phagocytes. The authors earlier demonstrated that alginates enriched in mannuronic acid stimulate human monocytes to produce high levels of cytokines such as tumour necrosis factor (TNF), IL-1, IL-6. In this study the authors have measured the TNF production from peripheral blood mononuclear cells (PBMC) in different groups of insulin-dependent diabetes mellitus (IDDM) patients after stimulation with different alginates and lipopolysaccharide (LPS). It was found that high G-alginate did not induce TNF production in any of the groups. High-M alginate and LPS induced a dose-dependent TNF production in all groups and the production was significantly different from unstimulated cells. The highest TNF response was found in newly diagnosed IDDM patients and the lowest was in the controls. |
| Databáze: | OpenAIRE |
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