Mesenchymal stem cells from human bone marrow or adipose tissue differently modulate mitogen-stimulated B-cell immunoglobulin production in vitro
| Autor: | Peter Tivchev, Dobroslav Kyurkchiev, Gabriel Elmadjian, Liubomir Tzvetanov, Iskra Altankova, Stanimir Kyurkchiev, Ivan Bochev |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Clinical uses of mesenchymal stem cells Adipose tissue Bone Marrow Cells Receptors Cell Surface Cell Separation Biology Immunophenotyping Colony-Forming Units Assay Antigens CD Osteogenesis medicine Humans Vimentin Cell Shape Cells Cultured B cell Aged Cell Proliferation Stem cell transplantation for articular cartilage repair Aged 80 and over B-Lymphocytes Adipogenesis Mesenchymal stem cell Endoglin Mesenchymal Stem Cells 3T3-L1 Cell Biology General Medicine Fibroblasts Middle Aged Cell biology medicine.anatomical_structure Adipose Tissue Antibody Formation Immunology Bone marrow Mitogens |
| Zdroj: | Cell Biology International. 32:384-393 |
| ISSN: | 1065-6995 |
| Popis: | Mesenchymal stem cells (MSC) have been characterized as multipotent cells which are able to differentiate into several mesodermal and nonmesodermal lineage cells and this feature along with their extensive growth and comprehensive immunomodulatory properties establish them as a promising tool for therapeutic applications, including cell-based tissue engineering and treatment of immune-mediated disorders. Although bone marrow (BM) is the most common MSC source, cells with similar characteristics have been shown to be present in several other adult tissues. Adipose tissue (AT), large quantities of which can be easily obtained, represents an attractive alternative to BM in isolating adipose tissue-derived MSC (AT-MSC). BM-MSCs and AT-MSCs share some immunomodulatory properties as they are both not inherently immunogenic and suppress the proliferation of alloantigen- or mitogen-stimulated T-cells. Our purpose was to comparatively examine under appropriate in vitro conditions, phenotypes, morphology and some functional properties of BM-MSCs and AT-MSCs, such as differentiation potential and especially the ability to suppress the immunoglobulin production by mitogen-stimulated B-cells. While the morphological, immunophenotypical, colony-forming and adipogenic characteristics of both types of cells were almost identical, AT-MSCs showed less potential for osteogenic differentiation than BM-MSCs. We found that AT-MSCs not only inhibited the Ig-production but also suppressed this B-cell function to a much greater extent compared to BM-MSC. This finding supports the potential role of AT-MSCs as an alternative to BM-MSCs for clinical purposes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text