Subpollens delivery of Platanus acerifolia pollen allergen Pla a3 and nucleic acid into lungs and cells
| Autor: | Zhang Wei, Zhou Shumin, Li Yang, Yan Fucheng, Peng Jiaxian, Zhang Zheng, Zhang Luying, Lin Yichun, Lu Senlin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Biophysics medicine.disease_cause Biochemistry Antibodies Proteaceae 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Allergen Pollen Nucleic Acids medicine Animals Humans DAPI Particle Size Molecular Biology Lung Sensitization Messenger RNA Mice Inbred BALB C biology Chemistry Cell Biology Pneumonia respiratory system Allergens Antigens Plant Molecular biology Staining Oxidative Stress 030104 developmental biology medicine.anatomical_structure Polyclonal antibodies A549 Cells 030220 oncology & carcinogenesis Nucleic acid biology.protein Female |
| Zdroj: | Biochemical and biophysical research communications. 513(4) |
| ISSN: | 1090-2104 |
| Popis: | Pollen allergy is a very serious seasonal respiratory disease. However, there has been a lack of understanding how pollen allergens enter the body and act on cells. This study focused on the release, transport and characteristic of Pla a3 allergen of the Platanus acerifolia pollen. Pla a3 protein was purified by prokaryotic expression system for preparation of polyclonal antibody. The distribution and release of Pla a3 protein in pollen were observed by immunohistochemistry. Mice were immunized with purified Pla a3 protein and SPPs, respectively. The pathological examination of mouse lung tissue proved that SPPs, as a fine particle in the range of 0.1-1μm, can enter the deep part of the lung directly through the respiratory tract and led to inflammation. Furthermore, DAPI staining confirmed a certain amount of nucleic acids in SPPs. After incubation with SPPs for 6 h, the Pla a3 mRNA could be detected in A549 cells by PCR. This suggests that nucleic acid wrapped in SPPs could be delivered into A549 cells. These results could provide a new clue and experimental data accumulation for further study on the mechanism of pollen sensitization. |
| Databáze: | OpenAIRE |
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