An altered intestinal mucosal microbiome in HIV-1 infection is associated with mucosal and systemic immune activation and endotoxemia
| Autor: | Davey M. Smith, Charles E. Robertson, Eric J. Lee, Gregory L. Austin, Z Dong, Cara C. Wilson, Basile Siewe, Sara Gianella, Daniel N. Frank, Alan L. Landay, Daniel K. Hecht, Cassandra V. Kotter, Stephanie M. Dillon |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Colon Biopsy Immunology Inflammation HIV Infections Lymphocyte Activation Medical and Health Sciences Article Microbiology Body Mass Index 03 medical and health sciences Young Adult Immune system Intestinal mucosa Immunity T-Lymphocyte Subsets Prevotella medicine Immunology and Allergy Humans Microbiome Intestinal Mucosa 030304 developmental biology 0303 health sciences biology 030306 microbiology Microbiota Dendritic cell Biodiversity Biological Sciences Middle Aged Viral Load biology.organism_classification medicine.disease Endotoxemia 3. Good health CD4 Lymphocyte Count Diet HIV-1 Dysbiosis Female medicine.symptom |
| Zdroj: | Mucosal immunology Dillon, SM; Lee, EJ; Kotter, CV; Austin, GL; Dong, Z; Hecht, DK; et al.(2014). An altered intestinal mucosal microbiome in HIV-1 infection is associated with mucosal and systemic immune activation and endotoxemia.. Mucosal immunology, 7(4), 983-994. doi: 10.1038/mi.2013.116. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/3bx2n419 |
| ISSN: | 1935-3456 |
| DOI: | 10.1038/mi.2013.116. |
| Popis: | Human immunodeficiency virus-1 (HIV-1) infection disrupts the intestinal immune system, leading to microbial translocation and systemic immune activation. We investigated the impact of HIV-1 infection on the intestinal microbiome and its association with mucosal T-cell and dendritic cell (DC) frequency and activation, as well as with levels of systemic T-cell activation, inflammation, and microbial translocation. Bacterial 16S ribosomal DNA sequencing was performed on colon biopsies and fecal samples from subjects with chronic, untreated HIV-1 infection and uninfected control subjects. Colon biopsies of HIV-1-infected subjects had increased abundances of Proteobacteria and decreased abundances of Firmicutes compared with uninfected donors. Furthermore at the genus level, a significant increase in Prevotella and decrease in Bacteroides was observed in HIV-1-infected subjects, indicating a disruption in the Bacteroidetes bacterial community structure. This HIV-1-associated increase in Prevotella abundance was associated with increased numbers of activated colonic T cells and myeloid DCs. Principal coordinates analysis demonstrated an HIV-1-related change in the microbiome that was associated with increased mucosal cellular immune activation, microbial translocation, and blood T-cell activation. These observations suggest that an important relationship exists between altered mucosal bacterial communities and intestinal inflammation during chronic HIV-1 infection. |
| Databáze: | OpenAIRE |
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