Cerebrospinal fluid cytotoxicity does not affect survival in amyotrophic lateral sclerosis
Autor: | Lucía Galán, Matilde Yáñez, Juan-Alberto Arranz-Tagarro, Jordi A. Matías-Guiu, Antonio G. García, A. Vela-Souto, Antonio Guerrero-Sola, Vanesa Pytel, Jorge Matías-Guiu, Ulises Gómez-Pinedo |
---|---|
Rok vydání: | 2016 |
Předmět: |
Adult
Male 0301 basic medicine Oncology medicine.medical_specialty medicine.medical_treatment Lower risk 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Internal medicine medicine Humans Amyotrophic lateral sclerosis Respiratory system Cytotoxicity Cells Cultured Aged Cerebrospinal Fluid Motor Neurons Proportional hazards model business.industry Amyotrophic Lateral Sclerosis General Medicine Middle Aged medicine.disease Gastrostomy 030104 developmental biology medicine.anatomical_structure Neurology Female Neurology (clinical) business Biomarkers 030217 neurology & neurosurgery Motor cortex |
Zdroj: | Acta Neurologica Scandinavica. 136:212-216 |
ISSN: | 0001-6314 |
Popis: | OBJECTIVES Cerebrospinal fluid (CSF) from some patients with amyotrophic lateral sclerosis (ALS) has been demonstrated to significantly reduce the neuronal viability of primary cell cultures of motor neurons. We aimed to study the potential clinical consequences associated with the cytotoxicity of CSF in a cohort of patients with ALS. METHODS We collected CSF from thirty-one patients with ALS. We analysed cytotoxicity by incubating it into the primary cultures of motor cortex neurons. Neural viability was quantified after 24 hours using the colorimetric MTT reduction assay. All patients were followed up from the moment of diagnosis to death, and a complete evaluation during disease progression and survival was performed, including gastrostomy and respiratory assistance. RESULTS Twenty-one patients (67.7%) presented a cytotoxic CSF. There were no significant differences between patients with and without cytotoxicity regarding mean time from symptom onset to the diagnosis, from the diagnosis to death, from the diagnosis to respiratory assistance with BIPAP, from diagnosis to gastrostomy and from the onset of symptoms to death. In Cox regression analysis, bulbar onset, but not cytotoxicity, gender or age at onset, was associated with a lower risk of survival. CONCLUSIONS Cerebrospinal fluid cytotoxicity was not associated with differential survival rates. This suggests that the presence of cytotoxicity in CSF, measured through neuronal viability in primary cultures of motor cortex neurons, could reflect different mechanisms of the disease, but it does not predict disease outcome. |
Databáze: | OpenAIRE |
Externí odkaz: |