Antiangiogenic activity of sophorolipids extracted from refined bleached deodorized palm olein

Autor:	Shazmin Kithur Mohamed, Amin Malik Shah Abdul Majid, Mansoureh Nazari, Syed Mahmood, Aman Shah Abdul Majid, Abdul Rashid M. Yatim, Muhammad Asif, Hussein M. Baharetha
Rok vydání:	2019
Předmět:	Vascular Endothelial Growth Factor A Antioxidant medicine.medical_treatment Angiogenesis Inhibitors Aorta Thoracic anticancer 030226 pharmacology & pharmacy Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement medicine Animals Humans Pharmacology (medical) 030212 general & internal medicine Food science Cytotoxicity Candida Pharmacology palm oil integumentary system sophorolipids food and beverages Yeast In vitro Rats Oleic acid Vegetable oil chemistry Antiangiogenic Fermentation Glycolipids Ex vivo Research Article Oleic Acid
Zdroj:	Indian Journal of Pharmacology
ISSN:	0253-7613
DOI:	10.4103/ijp.ijp_312_18
Popis:	OBJECTIVES: Sophorolipids (SLs) are a group of surface-active glycolipids produced by a type of nonpathogenic yeast Candida bombicola in the presence of vegetable oil through fermentation technology. SLs have shown antitumor activity; however, the mechanism of action underlying the anticancer activity of SLs is poorly understood. This work evaluated the anticancer activity of SLs fermented from palm oil by exploring its antiangiogenic activity. MATERIALS AND METHODS: The SLs that were fermented and further characterized for their biochemical activities. Cytotoxicity study was performed to assess cytostatic properties. A series of in vitro and ex vivo angiogenesis assay was also carried out. The relative fold change in the expression of p53 mRNA by SLs was also studied. RESULTS: Altogether, the data show that SLs derived from palm oil fermentation process inhibited neovascularization in the ex vivo tissue segments and also the endothelial cell proliferation between 50% and 65% inhibition as a whole. The palm oil derived SLs also caused downregulation of the suppression level of vascular endothelial growth factor and also upregulate the p53 mRNA level. The analytical studies revealed the presence of high amount of phenolic compounds but with relatively weak antioxidant activity. The gas chromatography-mass spectrometry studies revealed abundant amount of palmitic and oleic acid, the latter an established antiangiogenic agent, and the former being proangiogenic. CONCLUSION: Therefore, it can be concluded from this study that SLs derived from fermented palm oil have potent antiangiogenic activity which may be attributed by its oleic acid component.
Databáze:	OpenAIRE
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