Chitosan based thermosensitive injectable hydrogels for controlled delivery of loxoprofen: development, characterization and in-vivo evaluation
| Autor: | Zahid Hussain, Mahmood Ahmad, Muhammad Sohail, Mubeen Kousar, Shahzeb Khan, Syed Ahmed Shah, Haroon Rashid, Sabeeh Mohsin, Muhammad Usman Minhas, Mudassir Abbasi, Umair Ahmad |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Thermogravimetric analysis
macromolecular substances 02 engineering and technology Biochemistry Chitosan 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems Differential scanning calorimetry Structural Biology In vivo Chlorocebus aethiops medicine Animals Vero Cells Molecular Biology 030304 developmental biology Drug Carriers 0303 health sciences Molecular Structure Phenylpropionates Viscosity Spectrum Analysis Anti-Inflammatory Agents Non-Steroidal Temperature technology industry and agriculture Hydrogels General Medicine Loxoprofen Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Controlled release Drug Liberation chemistry Rabbits Glutaraldehyde Swelling medicine.symptom Rheology 0210 nano-technology Nuclear chemistry medicine.drug |
| Zdroj: | International Journal of Biological Macromolecules. 129:233-245 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.02.031 |
| Popis: | Oral drug delivery is natural, most acceptable and desirable route for nearly all drugs, but many drugs like NSAIDs when delivered by this route cause gastrointestinal irritation, gastric bleeding, ulcers, and many undesirable effects which limits their usage by oral delivery. Moreover, it is almost impossible to control the release of a drug in a targeted location in body. We developed thermo-responsive chitosan-co-poly(N-isopropyl-acrylamide) injectable hydrogel as an alternative for the gastro-protective and controlled delivery of loxoprofen sodium as a model drug. A free radical polymerization technique was used to synthesize thermo-responsive hydrogel by cross-linking chitosan HCl with NIPAAM using glutaraldehyde as cross-linker. Confirmation of crosslinked hydrogel structure was done by Fourier transform infrared spectra (FTIR). The thermal stability of hydrogel was confirmed through thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The scanning electron microscopy (SEM) was performed to evaluate the structural morphology of cross-linked hydrogel. To evaluate the rheological behavior of hydrogel with increasing temperature, rheological study was performed. Swelling and in vitro drug release studies were carried out under various temperature and pH conditions. The swelling study revealed that maximum swelling was observed at low pH (pH 1.2) and low temperature (25 °C) compared to the high range of pH and temperature and it resulted in quick release of the drug. The high range of pH (7.4) and temperature (37 °C) however caused controlled release of the drug. The in vivo evaluation of the developed hydrogel in rabbits demonstrated the controlled release behavior of fabricated system. |
| Databáze: | OpenAIRE |
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