Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon
| Autor: | Silvia Cristina da Silva Pedroso, Ismari Perini Furlaneto, Alex Junior Souza de Souza, Darlene Kássia Saraiva Queiroz Pantoja, Washington Luiz Assunção Pereira, Yan Corrêa Rodrigues, Karla Valéria Batista Lima |
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| Rok vydání: | 2021 |
| Předmět: |
Tuberculosis
Buffaloes Zoonoses Bacterianas Microbiology 03 medical and health sciences Zoonoses Virology parasitic diseases medicine Animals Humans Tuberculose Bovina / transmiss?o 0303 health sciences Mycobacterium bovis biology 030306 microbiology Amazon rainforest Zoonosis 0402 animal and dairy science Inspe??o Sanit?ria 04 agricultural and veterinary sciences General Medicine Mycobacterium bovis / patogenicidade Vigil?ncia em Sa?de P?blica biology.organism_classification medicine.disease 040201 dairy & animal science Infec??es por Mycobacterium / veterin?ria Infectious Diseases Mycobacterium tuberculosis complex Granuloma Tuberculose / veterin?ria Parasitology Bubalus Abattoirs Brazil Mycobacterium |
| Zdroj: | Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| ISSN: | 1972-2680 |
| Popis: | This work was supported by the Instituto Evandro Chagas, Secretaria de Vigil?ncia em Sa?de, Minist?rio da Sa?de. Funda??o de Amparo ? Pesquisa do Par?/Universidade do Estado do Par? (FAPESPA/UEPA) [Cooperation grant N? 004/2019]. Federal University of Par?. Post-graduate Program in Animal Science. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura Celular e Citogen?tica. Ananindeua, PA, Brasil / State University of Par?. Post-graduate Program in Parasitic Biology in the Amazon. Bel?m, PA, Brazil. State University of Par?. Post-graduate Program in Parasitic Biology in the Amazon. Bel?m, PA, Brazil / University Center of Par?. Post-graduate Program in Health Education - Medical Education. Bel?m, PA, Brazil. State University of Par?. Post-graduate Program in Parasitic Biology in the Amazon. Bel?m, PA, Brazil. Federal Rural University of Amazonia. Post-graduate Program in Animal Health and Production in the Amazon. Bel?m, PA, Brazil. Max Planck University Center. Indaiatuba, SP, Brazil. Federal Rural University of Amazonia. Institute of Animal Health and Production. Bel?m, PA, Brazil. Introduction: Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon region. Methodology: Granulomatous lesions suggestive of TB obtained from 109 buffaloes (n =109) during sanitary inspection at slaughter were subjected to histopathological evaluation, immunohistochemical (IHC) detection of Mycobacterium antigens, and to molecular tests (PCR) to detect hsp65, IS6110 and RD4 genes, which are specific to Mycobacterium spp., Mycobacterium tuberculosis Complex (MTBC) and M. bovis, respectively. Results: PCR results indicated Mycobacterium infection in 87.2% of the cases, of which 69.5% were positive for M. bovis, 27.4% belonged to MTBC, and 3.1% were probably non-TB mycobacteria. There was good agreement between the genus-specific molecular technique and the histopathological analysis. This high frequency of TB cases caused by non-M. bovis suggests a diversified scenario of mycobacteria associated with bubaline TB in the Brazilian Amazon region.Conclusions: The results reinforce the need of discussing the inclusion of more accurate techniques in examinations carried out by Inspection Services in Brazil |
| Databáze: | OpenAIRE |
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