Inhibitory role of oxytocin on TNFα expression assessed in vitro and in vivo
| Autor: | Sarah Garrido-Urbani, Françoise Rohner-Jeanrenaud, Jordi Altirriba, Anne-Laure Poher, Patricia Ropraz, Nicolas Deblon, Aurélie Caillon |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Adipose tissue macrophages Macrophage polarization Adipose tissue Bone Marrow Cells ddc:616.07 Biology Oxytocin 03 medical and health sciences Mice Endocrinology Internal medicine Internal Medicine medicine Animals ddc:616 Inflammation Tumor Necrosis Factor-alpha Macrophages Body Weight General Medicine M2 Macrophage 030104 developmental biology Cytokine Adipose Tissue Body Composition Cytokine secretion Macrophage proliferation hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Diabetes & Metabolism (2017) |
| ISSN: | 1878-1780 1262-3636 |
| Popis: | Aim Oxytocin administration to diet-induced obese (DIO) rodents, monkeys and humans decreases body weight and fat mass with concomitant improvements in glucose metabolism. Moreover, several studies show an immunomodulatory role of oxytocin in a number of settings (such as atherosclerosis, injury, sepsis). This study aims to shed some light on the effects of oxytocin on macrophage polarization and cytokine production, as well as its possible impact on these parameters in adipose tissue in DIO mice with impaired glucose metabolism. Methods Mouse bone marrow cells were differentiated into macrophages and treated with oxytocin. Macrophage proliferation, cytokine secretion and macrophage populations were determined. For experiments in vivo , DIO mice were treated with oxytocin for 2 weeks. Body weight and composition and glucose tolerance were subsequently followed. At the end of treatment, adipose tissue macrophage populations, plasma cytokine levels and cytokine expression in adipose tissue were determined. Results In bone marrow-derived macrophages, oxytocin induced an anti-inflammatory phenotype (decreased M1/M2 ratio). In M1-derived macrophages, oxytocin decreased TNFα secretion, with no effects on the other cytokines tested nor any effect on cytokine secretion by M2-derived macrophages. Oxytocin treatment in DIO mice in vivo led to decreased body weight accompanied by an improvement in glucose tolerance, with no changes in plasma cytokine levels. In adipose tissue, oxytocin decreased Tnfα expression without modifying the M1/M2 macrophage ratio. Conclusion Oxytocin treatment decreases TNFα production both in vitro (in bone marrow-derived macrophages) and in vivo (in epididymal adipose tissue) in DIO mice. This effect may also be contributory to the observed improvement in glucose metabolism. |
| Databáze: | OpenAIRE |
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