Recombinant subtype A and B human respiratory syncytial virus clinical isolates co-infect the respiratory tract of cotton rats
Autor: | Emma L Millar, Grace Y Olinger, Linda J. Rennick, Nicholas A. Crossland, Sham Nambulli, Ken Lemon, W. Paul Duprex |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Genotype Green Fluorescent Proteins Respiratory System 030106 microbiology Respiratory Mucosa Respiratory Syncytial Virus Infections Virus Cell Line law.invention 03 medical and health sciences Antigen law Virology medicine Animals Humans Sigmodontinae Cotton rat Lung Respiratory Tract Infections biology Coinfection biology.organism_classification medicine.disease Reverse Genetics Reverse genetics Luminescent Proteins 030104 developmental biology medicine.anatomical_structure Bronchiolitis Respiratory Syncytial Virus Human Viral evolution Recombinant DNA Female Respiratory tract |
Zdroj: | Journal of General Virology. 101:1056-1068 |
ISSN: | 1465-2099 0022-1317 |
Popis: | Human respiratory syncytial virus (HRSV) is an important respiratory pathogen causing a spectrum of illness, from common cold-like symptoms, to bronchiolitis and pneumonia requiring hospitalization in infants, the immunocompromised and the elderly. HRSV exists as two antigenic subtypes, A and B, which typically cycle biannually in separate seasons. There are many unresolved questions in HRSV biology regarding the interactions and interplay of the two subtypes. Therefore, we generated a reverse genetics system for a subtype A HRSV from the 2011 season (A11) to complement our existing subtype B reverse genetics system. We obtained the sequence (HRSVA11) directly from an unpassaged clinical sample and generated the recombinant (r) HRSVA11. A version of the virus expressing enhanced green fluorescent protein (EGFP) from an additional transcription unit in the fifth (5) position of the genome, rHRSVA11EGFP(5), was also generated. rHRSVA11 and rHRSVA11EGFP(5) grew comparably in cell culture. To facilitate animal co-infection studies, we derivatized our subtype B clinical isolate using reverse genetics toexpress the red fluorescent protein (dTom)-expressing rHRSVB05dTom(5). These viruses were then used to study simultaneous in vivo co-infection of the respiratory tract. Following intranasal infection, both rHRSVA11EGFP(5) and rHRSVB05dTom(5) infected cotton rats targeting the same cell populations and demonstrating that co-infection occurs in vivo. The implications of this finding on viral evolution are important since it shows that inter-subtype cooperativity and/or competition is feasible in vivo during the natural course of the infection. |
Databáze: | OpenAIRE |
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