Genetically engineered bacteriophage delivers a tumor necrosis factor alpha antagonist coating on neural electrodes
| Autor: | Youngjun Kim, Georgette B. Salieb-Beugelaar, Young-Hyun Jin, Thomas Stieglitz, Chang-Hoon Nam |
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| Rok vydání: | 2014 |
| Předmět: |
Materials science
Stereochemistry Cell Survival Surface Properties Amino Acid Motifs Molecular Sequence Data Biomedical Engineering Anti-Inflammatory Agents Bioengineering Apoptosis engineering.material Dip-coating law.invention Biomaterials Drug Delivery Systems Coating law Cell Line Tumor Fluorescence microscope Platinum binding Humans Bacteriophages Electrodes Platinum biology Base Sequence Tumor Necrosis Factor-alpha Viscosity Quartz crystal microbalance DNA biology.organism_classification Elasticity Surface coating Filamentous bacteriophage Microscopy Fluorescence Recombinant DNA engineering Biophysics Genetic Engineering Peptides |
| Zdroj: | Biomedical materials (Bristol, England). 9(1) |
| ISSN: | 1748-605X |
| Popis: | This paper reports a novel approach for the formation of anti-inflammatory surface coating on a neural electrode. The surface coating is realized using a recombinant f88 filamentous bacteriophage, which displays a short platinum binding motif and a tumor necrosis factor alpha antagonist (TNF-α antagonist) on p3 and p8 proteins, respectively. The recombinant bacteriophages are immobilized on the platinum surface by a simple dip coating process. The selective and stable immobilization of bacteriophages on a platinum electrode is confirmed by quartz crystal microbalance with dissipation monitoring, atomic force microscope and fluorescence microscope. From the in vitro cell viability test, the inflammatory cytokine (TNF-α) induced cell death was prevented by presenting recombinant bacteriophage coating, albeit with no significant cytotoxic effect. It is also observed that the bacteriophage coating does not have critical effects on the electrochemical properties such as impedance and charge storage capacities. Thus, this approach demonstrates a promising anti-apoptotic as well as anti-inflammatory surface coating for neural implant applications. |
| Databáze: | OpenAIRE |
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