UV cross-linking of donor corneas confers resistance to keratolysis
| Autor: | Claes H. Dohlman, Samer N. Arafat, Anita Shukla, James Chodosh, Marie-Claude Robert, Joseph B. Ciolino |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Stromal cell Photosensitizing Agents Chemistry Extramural Ultraviolet Rays Corneal Stroma Riboflavin Molecular biology eye diseases Tissue Donors Article Surgery Corneal Diseases Cornea Ophthalmology Cross-Linking Reagents Collagen metabolism medicine Humans sense organs Collagen Collagenases Ex vivo Enzymatic degradation |
| Zdroj: | Cornea. 33(9) |
| ISSN: | 1536-4798 |
| Popis: | The aim of this study was to develop a modified ex vivo corneal cross-linking method that increases stromal resistance to enzymatic degradation for use as a carrier for the Boston keratoprosthesis.Ex vivo cross-linking of human corneas was performed using Barron artificial anterior chambers. The corneas were deepithelialized, pretreated with riboflavin solution (0.1% riboflavin/20% dextran), and irradiated with ultraviolet A (UV-A) light (λ = 370 nm, irradiance = 3 mW/cm) for various durations. The combined effect of UV-A and gamma (γ) irradiation was also assessed using the commercially available γ-irradiated corneal donors. The corneas were then trephined and incubated at 37°C with 0.3% collagenase A solution. The time to dissolution of each cornea was compared across treatments.Deepithelialized corneas (no UV light, no riboflavin) dissolved in 5.8 ± 0.6 hours. Cross-linked corneas demonstrated increased resistance to dissolution, with a time to dissolution of 17.8 ± 2.6 hours (P0.0001). The corneal tissues' resistance to collagenase increased with longer UV-A exposure, reaching a plateau at 30 minutes. Cross-linking both the anterior and posterior corneas did not provide added resistance when compared with cross-linking the anterior corneas only (P0.05). γ-irradiated corneas dissolved as readily as deepithelialized controls regardless of whether they were further cross-linked (5.6 ± 1.2 hours) or not (6.1 ± 0.6 hours) (P = 0.43).Collagen cross-linking of the deepithelialized anterior corneal surface for 30 minutes conferred optimal resistance to in vitro keratolysis by collagenase A. |
| Databáze: | OpenAIRE |
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