Synapsis-Defective Mutants Reveal a Correlation Between Chromosome Conformation and the Mode of Double-Strand Break Repair During Caenorhabditis elegans Meiosis
| Autor: | Andreas Eizinger, Allison Hurlburt, Sarit Smolikov, Eric J Rogers, Anne M. Villeneuve, Monica P. Colaiácovo |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Recombination Genetic Genetics DNA Repair Synaptonemal Complex DNA repair Mutant Synapsis Investigations Biology Genetic recombination Double Strand Break Repair Chromosomal crossover Chromosome Pairing Meiosis Synaptonemal complex Mutation Animals DNA Breaks Double-Stranded Female Crossing Over Genetic Caenorhabditis elegans Genes Helminth |
| Zdroj: | Genetics. 176:2027-2033 |
| ISSN: | 1943-2631 |
| DOI: | 10.1534/genetics.107.076968 |
| Popis: | SYP-3 is a new structural component of the synaptonemal complex (SC) required for the regulation of chromosome synapsis. Both chromosome morphogenesis and nuclear organization are altered throughout the germlines of syp-3 mutants. Here, our analysis of syp-3 mutants provides insights into the relationship between chromosome conformation and the repair of meiotic double-strand breaks (DSBs). Although crossover recombination is severely reduced in syp-3 mutants, the production of viable offspring accompanied by the disappearance of RAD-51 foci suggests that DSBs are being repaired in these synapsis-defective mutants. Our studies indicate that once interhomolog recombination is impaired, both intersister recombination and nonhomologous end-joining pathways may contribute to repair during germline meiosis. Moreover, our studies suggest that the conformation of chromosomes may influence the mode of DSB repair employed during meiosis. |
| Databáze: | OpenAIRE |
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