Glycosylate and move! The glycosyltransferase Maf is involved in bacterial flagella formation

Autor: François Alberto, Régine Lebrun, Véronique Roig-Zamboni, Nao Yamakawa, Gerlind Sulzenbacher, Yann Guérardel, Xin-Xin Qian, Yves Bourne, Long-Fei Wu, Pascal Mansuelle, Dorothée Murat, Renaud Vincentelli
Přispěvatelé: Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Plateforme Protéomique [Marseille], Institut de Microbiologie de la Méditerranée (IMM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), ANR-10-BLAN-1320,MagneticFlAp,Mécanismes d'assemblage et de fonctionnement de l'appareillage flagellaire des bactéries magnétotactiques(2010), ANR-10-INBS-05-01/10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010), Université de Lille-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Glycan
Glycosylation
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
030106 microbiology
Flagellum
Microbiology
Campylobacter jejuni
[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Glycosyltransferase
Magnetospirillum
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Ecology
Evolution
Behavior and Systematics
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
biology
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
Glycosyltransferases
Sugar Acids
biology.organism_classification
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Sialic acid
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
chemistry
Biochemistry
Flagella
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Sialic Acids
biology.protein
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Bacteria
Flagellin
Zdroj: Environmental Microbiology
Environmental Microbiology, Wiley-Blackwell, 2018, 20 (1), pp.228-240. ⟨10.1111/1462-2920.13975⟩
Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2018, 20 (1), pp.228-240. ⟨10.1111/1462-2920.13975⟩
Environmental Microbiology, 2018, 20 (1), pp.228-240. ⟨10.1111/1462-2920.13975⟩
ISSN: 1462-2912
1462-2920
Popis: International audience; The flagella of various Gram-negative bacteria are decorated with diverse glycan structures, amongst them nonulosonic acids related to the sialic acid family. Although nonulosonic sugar biosynthesis pathways have been dissected in various pathogens, the enzymes transferring the sugars onto flagellin are still poorly characterized. The deletion of genes coding for motility associated factors (Mafs) found in many pathogenic strains systematically gives rise to nonflagellated bacteria lacking specific nonulosonic sugars on the flagellins, therefore, relating Maf function to flagellin glycosylation and bacterial motility. We investigated the role of Maf from our model organism, Magnetospirillum magneticum AMB-1, in the glycosylation and formation of the flagellum. Deletion of the gene amb0685 coding for Maf produced a nonflagellated bacterium where the flagellin was still produced but no longer glycosylated. Our X-ray structure analysis revealed that the central domain of Maf exhibits similarity to sialyltransferases from Campylobacter jejuni. Glycan analysis suggested that the nonulosonic carbohydrate structure transferred is pseudaminic acid or a very close derivative. This work describes the importance of glycosylation in the formation of the bacterial flagellum and provides the first structural model for a member of a new bacterial glycosyltransferase family involved in nonulosonic acids transfer onto flagellins.
Databáze: OpenAIRE
Externí odkaz: