Highly sensitive assay for the measurement of serotonin in microdialysates using capillary high-performance liquid chromatography with electrochemical detection
| Autor: | Sandrine Parrot, Luc Denoroy, Laura Lambás-Señas, Bernard Renaud, Sabine Sentenac |
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| Rok vydání: | 2007 |
| Předmět: |
Detection limit
Serotonin Microdialysis Chromatography Capillary action Metabolite Clinical Biochemistry Cell Biology General Medicine Electrochemical detection Hydroxyindoleacetic Acid Sensitivity and Specificity Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Electrochemistry Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B. 850:303-309 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2006.11.045 |
| Popis: | A highly sensitive isocratic capillary high-performance liquid chromatographic (HPLC) method with electrochemical detection (ED) for the simultaneous measurement of serotonin (5-hydroxytryptamine, 5-HT) and its metabolite 5-hydroxyindole-3-acetic acid (5-HIAA) in microdialysates has been developed using a 0.5 mm i.d. capillary column and a 11-nL detection cell. This method, validated on both pharmacological and analytical bases, can be performed using injection volumes as low as 1 microL. The limits of detection were 5.6 x 10(-11)mol/L and 3.0 x 10(-9)mol/L for 5-HT and 5-HIAA. Several applications of the present method are given on microdialysates from rodent brain and human spinal cord. |
| Databáze: | OpenAIRE |
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