Ketamine does not affect intestinal microcirculation in pentobarbital-anaesthetized rats during experimental endotoxaemia
| Autor: | Konrad Meissner, M Fogliata, Ch Lehmann, Michael Wendt, Th Feyerherd, Dragan Pavlovic, Matthias Gründling, Taras I. Usichenko, F. Feyerherd |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Pentobarbital Necrosis Lipopolysaccharide Endothelium Blood Pressure Pharmacology chemistry.chemical_compound medicine Animals Ketamine Escherichia coli Infections Anesthetics Analgesics General Veterinary business.industry Microcirculation Interleukin Endotoxemia Rats Intestines medicine.anatomical_structure chemistry Rats Inbred Lew Anesthesia Anesthesia Intravenous Cytokines Animal Science and Zoology Tumor necrosis factor alpha medicine.symptom business Intravital microscopy medicine.drug |
| Zdroj: | Laboratory Animals. 41:55-62 |
| ISSN: | 1758-1117 0023-6772 |
| Popis: | The objective of the study was to evaluate the effects of ketamine on intestinal microcirculation in pentobarbital-anaesthetized rats during experimental endotoxaemia. A prospective, randomized, controlled study was carried out using 32 male Lewis rats. The animals were divided into four groups ( n = 8 each). All animals were initially anaesthetized with 60 mg/kg pentobarbital (i.p.). Group 1 served as a control (18.5 mg/kg/h pentobarbital i.v.). Groups 2 and 4 received an endotoxin intravenous infusion of 15 mg/kg lipopolysaccharide (LPS) from Escherichia coli. Groups 3 and 4 also received 10 mg/kg/h ketamine (i.v.). After 2 h of observation, the animals were examined for intestinal functional capillary density (FCD) and leukocyte adherence to the venular endothelium by means of intravital fluorescence microscopy (IVM). Subsequent to this examination, blood samples were collected to determine release of the cytokines tumour necrosis factor (TNF)- α, interleukin (IL)-1 β, IL-6 and IL-10. Endotoxaemia tended to decrease intestinal FCD (mucosa: −10.1%, muscularis longitudinalis: −2%, muscularis circularis: −9.8%) and significantly increase leukocyte adherence within submucosal venules (collecting venules: +133%, postcapillary venules: +207%; P |
| Databáze: | OpenAIRE |
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