Chrysin mitigated obesity by regulating energy intake and expenditure in rats
| Autor: | Renuka Munshi, Sarayu A. Pai, Malvika Gursahani, Archana R. Juvekar, Snehal N. Mestry, Elvis A. F. Martis |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
VC vehicle control BMI body mass index OFTT oral fat tolerance test Flavonoid 0211 other engineering and technologies Adipose tissue lcsh:Medicine 02 engineering and technology Orli orlistat 01 natural sciences AUC area under the curve chemistry.chemical_compound Internal medicine 021105 building & construction medicine Pancreatic lipase PL pancreatic lipase Chrysin NC normal control HFD high fructose diet C100 chrysin 100 mg/kg p.o. body weight chemistry.chemical_classification AC/TC ratio-abdominal circumference to thoracic circumference ratio Sucrose preference Cholesterol lcsh:R p-NPP p-nitrophenyl palmitate TG triglycerides Propolis medicine.disease Obesity C25 chrysin 25 mg/kg p.o. body weight SEM standard error of mean 0104 chemical sciences 010404 medicinal & biomolecular chemistry Orlistat Endocrinology C50 chrysin 50 mg/kg p.o. body weight Complementary and alternative medicine chemistry Original Article Body mass index Locomotion GLP 1-glucagon like peptide 1 medicine.drug |
| Zdroj: | Journal of Traditional and Complementary Medicine, Vol 10, Iss 6, Pp 577-585 (2020) Journal of Traditional and Complementary Medicine |
| ISSN: | 2225-4110 |
| DOI: | 10.1016/j.jtcme.2019.09.002 |
| Popis: | Background and aim Chrysin is a flavonoid found in plant extracts from Passiflora species, honey and propolis. It has demonstrated anti-adipogenic activity in vitro but there are no studies substantiating the anti-obesity activity of chrysin in vivo. Experimental procedure The pancreatic lipase (PL) inhibitory potential of chrysin was determined by preliminary in silico screening and further confirmed by in vitro PL inhibitory assay and oral fat tolerance test (OFTT). The effect of chrysin on acute feed intake and sucrose preference test was determined in normal rats. Obesity was induced by feeding of high fructose diet (HFD) to the rats. The rats were divided into six groups: normal control, HFD control, orlistat and three doses of chrysin (25, 50 and 100 mg/kg body weight). Body weight, body mass index (BMI), abdominal circumference/thoracic circumference (AC/TC) ratio, calorie intake, adiposity index, fecal cholesterol, locomotor activity and histopathology of the adipose tissue of the rats were evaluated. Results Chrysin showed good affinity to PL with competitive type of inhibition. It significantly reduced serum triglycerides in OFTT. Chrysin also significantly reduced acute feed intake and sucrose preference in rats. Chrysin significantly decreased the body weight, BMI, AC/TC ratio, adiposity index, calorie intake while it significantly increased the fecal cholesterol and locomotor activity of the rats. Chrysin was found to reduce the size of the adipocytes when compared to the HFD control group. Conclusion Thus, chrysin exerted anti-obesity effect by inhibiting PL, reducing sucrose preference, reducing calorie intake and increasing the locomotor activity of rats. Graphical abstract Image 1021 Highlights • Chrysin was found to be competitive inhibitor of pancreatic lipase. • Chrysin reduced the body weight of rats. • Chrysin reduced calorie intake of rats. • Chrysin increased the locomotor activity of obese rats. • Chrysin reduced the hypertrophy of adipocytes. |
| Databáze: | OpenAIRE |
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