Intranasal immunization with live attenuated influenza vaccine plus chitosan as an adjuvant protects mice against homologous and heterologous virus challenge
| Autor: | Wenjie Zhang, Hanzhong Wang, Yahong Ding, Ze Chen, Jian Luo, Tao Zhang, Mei Zheng, Yinglei Yi, Chaoyang Dai, Fen Liu, Bing Sun, Xueliang Wang, Dan Zheng |
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| Rok vydání: | 2012 |
| Předmět: |
Influenza vaccine
Cross Protection medicine.medical_treatment Population Heterologous macromolecular substances Biology Antibodies Viral Vaccines Attenuated Virus Microbiology Interferon-gamma Mice Adjuvants Immunologic Orthomyxoviridae Infections Virology medicine Animals Live attenuated influenza vaccine education Administration Intranasal Chitosan Mice Inbred BALB C education.field_of_study Immunogenicity Vaccination General Medicine Influenza Vaccines Female Adjuvant |
| Zdroj: | Archives of Virology. 157:1451-1461 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Our previous studies have proven the adjuvanticity of chitosan in mice when administered with inactivated and subunit influenza vaccine. In this study, we investigated the adjuvant effect of chitosan on the immunogenicity and protective efficacy of a live attenuated influenza vaccine. Mice were inoculated intranasally with live attenuated influenza vaccine plus chitosan and then challenged with a high, lethal dose of homologous or heterologous virus. Antibody responses, secretion of IFN-γ by spleen cells, body weight loss, survival rates, and residual lung virus titers were tested. The results demonstrated that live attenuated influenza vaccine with chitosan adjuvant not only protected mice completely against challenge with the homologous virus but also provided good cross-protection against a heterologous virus. In addition, chitosan as adjuvant could significantly increase the levels of antigen-specific antibodies and the population of IFN-γ-secreting T cells. These results reveal the potential of chitosan as a candidate adjuvant for use in a live attenuated influenza vaccine. |
| Databáze: | OpenAIRE |
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