Detection of intestinal flora-derived bacterial antigen complexes in splenic macrophages of rats
| Autor: | I. S. Klasen, Marie-José Melief, G M Bahr, H. de Visser, W. B. Van Den Berg, C G Van Helden-Meeuwsen, M Y Gerrits-Boeye, J. Kool, L. M. C. Van Lieshout, J. G. H. Ruseler-Van Embden |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Flora Histology medicine.drug_class Enzyme-Linked Immunosorbent Assay Peptidoglycan Monoclonal antibody Microbiology Mice Antibody Specificity Polysaccharides medicine Animals Cecum Antigens Bacterial Mice Inbred BALB C Eubacterium Chemistry Macrophages Age Factors Antibodies Monoclonal Immunohistochemistry Rats Intestines Rats Inbred Lew Female Bacterial antigen Anatomy Spleen |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 42:1435-1441 |
| ISSN: | 1551-5044 0022-1554 |
| Popis: | We studied the presence of bacterial antigens in rat tissues. We produced a monoclonal antibody (MAb 2E9) directed against intestinal flora-derived peptidoglycan-polysaccharide complexes from human and rat feces. With several immunological techniques, the specificity of 2E9 for this bacterial product was demonstrated. Using 2E9 in an immunohistological assay, we were able to show the presence of bacterial products in macrophages in the red pulp of spleens of conventional Lewis rats. However, we found no correlation between the development of the intestinal flora and positive spleen staining with MAb 2E9. The results were confirmed by immunohistology with a previously described MAb 2-4 directed to muramyl dipeptide. Other lymphoid organs did not stain positively with 2E9 and 2-4. Neonatal and young rats showed no staining of the spleen, but positivity could be induced by injecting peptidoglycan-polysaccharide complexes systemically. We conclude that bacterial fragments are present in splenic macrophages of conventional rats. |
| Databáze: | OpenAIRE |
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