OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice
| Autor: | Soon-Wook Kwon, Xinglin Du, Tao Wu, Zhao Li, Wenzhu Jiang, Hongjia Zhang, Ziming Ma, Kai Huang, Na-Eun Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Zinc finger transcription factor
biology Abiotic stress OsGATA16 Soil Science food and beverages Plant culture Plant Science biology.organism_classification Cold tolerance Cell biology SB1-1110 chemistry.chemical_compound chemistry Seedling Transcription (biology) Cytokinin Haplotype GATA transcription factor Rice Transcription factor Agronomy and Crop Science Gene |
| Zdroj: | Rice, Vol 14, Iss 1, Pp 1-15 (2021) |
| ISSN: | 1939-8433 1939-8425 |
| Popis: | BackgroundCold stress is the main abiotic stress in rice, which seriously affects the growth and yield of rice. Identification of cold tolerance genes is of great significance for rice to solve these problems. GATA-family transcription factors involve diverse biological functions, however, their role in cold tolerance in rice remains unclear.ResultsIn this study, a GATA-type zinc finger transcription factorOsGATA16, which can improve cold tolerance, was isolated and characterized from rice. OsGATA16 belongs to OsGATA subfamily-II and contains 11 putative phosphorylation sites, a nuclear localization signal (NLS), and other several conserved domains.OsGATA16was expressed in all plant tissues, with the strongest in panicles. It was induced by cold and ABA treatments, but was repressed by drought, cytokinin and JA, and acted as a transcriptional suppressor in the nucleus. Overexpression ofOsGATA16improves cold tolerance of rice at seedling stage. Under cold stress treatments, the transcription of four cold-related genesOsWRKY45–1,OsSRFP1,OsCYL4, andOsMYB30was repressed inOsGATA16-overexpressing (OE) rice compared with wild-type (WT). Interestingly, OsGATA16 bound to the promoter ofOsWRKY45–1and repressed its expression.In addition, haplotype analysis showed thatOsGATA16polarized between the two major rice subspeciesjaponicaandindica, and had a non-synonymous SNP8 (336G) associated with cold tolerance.ConclusionOsGATA16 is a GATA transcription factor, which improves cold tolerance at seedling stage in rice. It acts as a positive regulator of cold tolerance by repressing some cold-related genes such asOsWRKY45–1,OsSRFP1,OsCYL4andOsMYB30. Additionally, OsGATA16 has a non-synonymous SNP8 (336G) associated with cold tolerance on CDS region. This study provides a theoretical basis for elucidating the mechanism of cold tolerance in rice and new germplasm resources for rice breeding. |
| Databáze: | OpenAIRE |
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