Good manufacturing practice-grade fibrin gel is useful as a scaffold for human mesenchymal stromal cells and supports in vitro osteogenic differentiation

Autor: Bruno Fiorentino, Mario Petrini, Stefano Guazzini, Manuela Scarpellini, Sara Galimberti, Simone Pacini, Rita Fazzi, Delfo D'Alessandro, Luisa Trombi
Rok vydání: 2008
Předmět:
Zdroj: Transfusion. 48:2246-2251
ISSN: 1537-2995
0041-1132
DOI: 10.1111/j.1537-2995.2008.01829.x
Popis: BACKGROUND: Recently, there has been an increased interest in using mesenchymal stromal cells (MSCs) in bone tissue engineering coupled with a suitable scaffold of both biological and synthetic origin. The cells and these constructs can be combined in vitro or directly in vivo to enhance tissue repair. MSCs are spindle-shaped cells capable of self-renewal and can be induced to differentiate mainly into osteo-, chondro-, and adipogenic-progeny types. Several biomaterials are currently available and, among them, fibrin-based constructs seem to be suitable for guiding the cells during tissue repair or regeneration due to their biocompatibility and biodegradability. STUDY DESIGN AND METHODS: Here, this study describes a simple in vitro system using human mesenchymal stromal cells (hMSCs) and fibrin scaffold prepared at different concentrations in fibrinogen (1.5%-3% and 6%) to evaluate cell proliferation and viability inside these constructs. RESULTS: The data demonstrate that the constructs with 3 percent in fibrinogen resulted in the best scaffolds, because within them the cells were able to proliferate and were uniformly distributed. Finally, analyzing the capability of the clots to support osteogenic differentiation of MSCs, we observed that they differentiated into osteoblasts. CONCLUSION: These results suggest that fibrin gel could be useful as a delivery system for hMSCs. B one regeneration is required for fracture healing, and different surgical and biological procedures have been used to promote osteogenesis. This process involves migration, proliferation, and differentiation of osteocompetent cells. However, successful repair of large bone defects remains a challenge for surgeons, especially in elderly patients or in patients with severe trauma. Moreover, in some clinical cases, the local recruitment of these cells is defective because the wound bed cannot provide them. Various strategies have been used to promote osteogenesis; among them tissue engineering seems to be increasingly promising for orthopedic therapeutic applications. It involves the use of cells coupled with biological or artificial matrices, which guide the cells during tissue repair or regeneration. These materials, loaded with osteocompetent cells, should be able to generate new
Databáze: OpenAIRE
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