Zika Virus Targets Multiple Tissues and Cell Types During the First Trimester of Pregnancy
| Autor: | Francisco Veas, Nabila Jabrane-Ferrat |
|---|---|
| Přispěvatelé: | Toulouse Institute for Infectious and Inflammatory Diseases, University of Toulouse, CNRS, INSERM, UPS, Toulouse, France. (Infinity), Hydrosciences Montpellier (HSM), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD), Kobinger G., Racine T. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
MESH: Zika Virus / metabolism [SDV]Life Sciences [q-bio] Umbilical cord Zika virus 0302 clinical medicine MESH: Macrophages / pathology MESH: Pregnancy MESH: Chlorocebus aethiops MESH: Placenta / pathology MESH: Zika Virus Infection / pathology MESH: Animals 030212 general & internal medicine Fetal placenta Maternal-fetal transmission MESH: Pregnancy Trimester First MESH: Organ Specificity ComputingMilieux_MISCELLANEOUS MESH: Primary Cell Culture / methods biology Umbilical cord mesenchymal stem cells MESH: Pregnancy Complications Infectious / virology MESH: Trophoblasts / pathology MESH: Umbilical Cord / virology 3. Good health Trophoblasts MESH: Infectious Disease Transmission Vertical medicine.anatomical_structure MESH: Trophoblasts / virology embryonic structures [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology MESH: Fetus / virology [SDV.IMM]Life Sciences [q-bio]/Immunology MESH: Macrophages / virology Cellular Tropism MESH: Zika Virus / pathogenicity MESH: Virus Cultivation / methods MESH: Vero Cells MESH: Viral Load / methods 03 medical and health sciences Placenta medicine MESH: Pregnancy Complications Infectious / pathology MESH: Specimen Handling / methods MESH: Tissue Distribution MESH: Umbilical Cord / pathology First trimester pregnancy Fetus Pregnancy MESH: Humans MESH: Zika Virus Infection / virology Macrophages Mesenchymal stem cell Trophoblast Maternal decidua basalis Zika virus infection MESH: Fetus / pathology biology.organism_classification medicine.disease Virology 030104 developmental biology Hofbauer cells MESH: Placenta / virology MESH: Pregnancy Complications Infectious / metabolism Decidual fibroblasts MESH: Female |
| Zdroj: | Zika Virus Zika Virus, 2142, Springer US, pp.235-249, 2020, Methods in Molecular Biology, ⟨10.1007/978-1-0716-0581-3_18⟩ Zika Virus : Methods and protocols Kobinger G.; Racine T. Zika Virus : Methods and protocols, 2142, Humana, pp.235-249, 2020, Methods in Molecular Biology, 978-1-0716-0580-6. ⟨10.1007/978-1-0716-0581-3_18⟩ Methods in Molecular Biology ISBN: 9781071605806 |
| DOI: | 10.1007/978-1-0716-0581-3_18⟩ |
| Popis: | International audience; The 2016 Zika virus (ZIKV) outbreak in the Americas has been characterized by an increased association frequency of fetal neuropathological abnormalities. To have a comprehensive and accurate knowledge of key elements of the clinically observed neurologic dysfunctions in Zika-infected babies, ZIKV transmission from mother to fetus needs to be deeply studied. Thus, it is important to determine the role of both virus-targeted cells and tissues within the mother-fetus interface. Cellular tropism and mechanisms of ZIKV transmission from mother to the fetus during early pregnancy still remain unknown on many aspects. To improve the characterization of the maternal-fetal ZIKV transmission, we have set up an ex vivo model using an organ culture approach with a light-invasive sampling from the first trimester of pregnancy samples. Thus, here we provide evidence that circulating epidemic ZIKV strains from Latin America widely target and destroy reproductive tissues, including the decidua basalis, fetal placenta, and umbilical cord. In addition, we show that ZIKV is able to differentially replicate in a large range of both maternal and fetal cells, including decidual fibroblasts and macrophages, fetal trophoblast and Hofbauer cells, as well as umbilical cord mesenchymal stem cells. This primary and broad ZIKV cellular tropism and the resulting abundant cytopathic-induced tissue effects during the first trimester of pregnancy show the upstream path of clinically observed congenital damages. |
| Databáze: | OpenAIRE |
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