Feline immunodeficiency virus can productively infect cultured endothelial cells from cat brain microvessels
| Autor: | Marie-Edith Lafon, Catherine A. Royer, Jean-Louis Gendrault, M. De Monte, A. Kirn, J P Gut, F. Koehren, Anne-Marie Steffan |
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| Rok vydání: | 1994 |
| Předmět: |
G2 Phase
Feline immunodeficiency virus Paclitaxel viruses Viral budding Gene Products gag Receptors Cell Surface Immunodeficiency Virus Feline Virus Replication Antigen Virology von Willebrand Factor medicine Animals Cells Cultured Syncytium biology Microglia Microcirculation Brain Lectin biology.organism_classification In vitro Lipoproteins LDL Endothelial stem cell Microscopy Electron medicine.anatomical_structure Culture Media Conditioned Receptors Mitogen Cats biology.protein Endothelium Vascular |
| Zdroj: | Journal of General Virology. 75:3647-3653 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Feline immunodeficiency virus (FIV) provokes a disease in cats characterized by histopathological lesions similar to those observed in AIDS patients. In order to determine whether endothelial cells from brain microvessels are involved in the central nervous system disease to the same extent as macrophages and microglia, cells were isolated from healthy cat brains, cultured and infected in vitro with the FIV Villefranche IFFA 1/88 strain. The isolated cells displayed typical endothelial cell ultrastructural features and were characterized further by von Willebrand factor-labelling and the binding of specific lectins such as Ulex europaeus lectin on their membrane. They were also able to take up acetylated low density lipoproteins. Two weeks after infection, significant amounts of FIV p24 antigen were detected by indirect immunofluorescence in syncytia and single cells. Concomitantly, the same antigen could be detected in the culture medium of the infected cells by an ELISA technique. Numerous viral particles as well as different steps in the process of viral budding were observed under transmission electron microscopy. The synthesis of FIV p24 antigens still occurred in cells in which replication was blocked in the G2 phase with taxol. Our results suggest the possibility of a productive infection of brain microvascular endothelial cells by FIV in vivo, which could lead to important perturbations in the functions of the blood-brain barrier. |
| Databáze: | OpenAIRE |
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