Determination of Orbifloxacin in Rabbit Plasma by High-Performance Liquid Chromatography with Fluorescence Detection
| Autor: | S. Rueda, M. A. Garcia, M. A. Bregante, J. J. Aramayona, C. Solans |
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| Rok vydání: | 1999 |
| Předmět: |
Detection limit
Chromatography Aqueous solution Chemistry Analytical chemistry General Medicine Sensitivity and Specificity High-performance liquid chromatography Fluorescence Fluorescence spectroscopy Analytical Chemistry Anti-Infective Agents Ciprofloxacin medicine Animals Rabbits Orbifloxacin Quantitative analysis (chemistry) Chromatography High Pressure Liquid medicine.drug Antibacterial agent |
| Zdroj: | Journal of Chromatographic Science. 37:199-202 |
| ISSN: | 1945-239X 0021-9665 |
| DOI: | 10.1093/chromsci/37.6.199 |
| Popis: | A simple and sensitive high-performance liquid chromatography method is developed for the determination of orbifloxacin (ORB) in rabbit plasma. Sample preparations are carried out by adding phosphate buffer (pH 7.4, 0.1 M) and extracting with trichloromethane. ORB and the internal standard, norfloxacin (NOR), are separated on a reversed-phase column using an aqueous phosphate buffer-acetonitrile (80:20, v/v) mobile phase. The concentrations of ORB and NOR eluting from the column with retention times of 2.16 and 3.09 min, respectively, are monitored by fluorescence detection at 338 (excitation) and 425 nm (emission). The method is shown to be linear from 4 to 1500 ng/mL (regression coefficient r2 = 0.999). The quantitation and detection limits are 4 and 9 ng/mL, respectively. Mean recovery is determined as 92% by the analysis of plasma standards containing 150, 750, and 1500 ng/mL. Inter- and intra-assay precisions were 4 and 3%, respectively. |
| Databáze: | OpenAIRE |
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