Role of glial cell derived neurotrophic factor in the protective effect of smilagenin on rat mesencephalic dopaminergic neurons damaged by MPP+

Autor: Yongfang Zhang, Yaer Hu, Antonia Orsi, Daryl Rees, Zongqin Xia
Rok vydání: 2008
Předmět:
medicine.medical_specialty
Glial Cell Line-Derived Neurotrophic Factor Receptors
Tyrosine 3-Monooxygenase
Neurite
Dopamine
Dopamine Agents
education
Biophysics
Biochemistry
Receptor tyrosine kinase
Rats
Sprague-Dawley
Mesencephalon
Structural Biology
Internal medicine
Smilagenin
mental disorders
Spirostans
Genetics
medicine
Glial cell line-derived neurotrophic factor
Animals
Glial Cell Line-Derived Neurotrophic Factor
GDNF receptor
Molecular Biology
Dopaminergic neuron
Neurons
Tyrosine hydroxylase
biology
urogenital system
Chemistry
Dopaminergic
Receptor Protein-Tyrosine Kinases
Cell Biology
Rats
Endocrinology
medicine.anatomical_structure
nervous system
Glial cell derived neurotrophic factor
1-Methyl-4-phenyl-1
2
3
6-tetrahydropyridine
Cytoprotection
biology.protein
Female
Neuron
GDNF family of ligands
medicine.drug
Zdroj: FEBS Letters. 582:956-960
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2008.02.039
Popis: Tyrosine hydroxylase immunohistochemical analysis revealed that in cultured mesencephalic dopaminergic neurons smilagenin (SMI), added prior to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPP+), protected against the drop of neuron number and neurite outgrowth length caused by MPP+. Addition of anti-GDNF and/or anti-GFR alpha 1 functional antibodies to the medium prior to SMI, eliminated mostly, though incompletely, the action of SMI. The expression of glial cell derived neurotrophic factor (GDNF) mRNA, but not GDNF receptor alpha1 (GFR alpha 1) or receptor tyrosine kinase mRNA in MPP+ intoxicated neurons was markedly elevated as early as 2h after the addition of SMI with a peak at 24-48 h. Therefore, an important route of the protective action of SMI on dopaminergic neurons is to stimulate intrinsic GDNF expression.
Databáze: OpenAIRE
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