CB1 Cannabinoid Receptor Inhibits Synaptic Release of Glutamate in Rat Dorsolateral Striatum
| Autor: | Gregory L. Gerdeman, David M. Lovinger |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Patch-Clamp Techniques
Cannabinoid receptor Physiology Morpholines Receptors Drug medicine.medical_treatment Glutamic Acid chemistry.chemical_element In Vitro Techniques Naphthalenes Calcium Synaptic Transmission Rats Sprague-Dawley Piperidines Rimonabant medicine Animals Dronabinol Patch clamp Receptors Cannabinoid Receptor Cannabinoids musculoskeletal neural and ocular physiology General Neuroscience Glutamate receptor Excitatory Postsynaptic Potentials Corpus Striatum Electric Stimulation Benzoxazines Rats nervous system chemistry Strontium Synapses Pyrazoles lipids (amino acids peptides and proteins) Cannabinoid Excitatory Amino Acid Antagonists Neuroscience Drug metabolism medicine.drug |
| Zdroj: | Journal of Neurophysiology. 85:468-471 |
| ISSN: | 1522-1598 0022-3077 |
| DOI: | 10.1152/jn.2001.85.1.468 |
| Popis: | CB1 cannabinoid receptors in the neostriatum mediate profound motor deficits induced when cannabinoid drugs are administered to rodents. Because the CB1 receptor has been shown to inhibit neurotransmitter release in various brain areas, we investigated the effects of CB1 activation on glutamatergic synaptic transmission in the dorsolateral striatum of the rat where the CB1 receptor is highly expressed. We performed whole cell voltage-clamp experiments in striatal brain slices and applied the CB1 agonists HU-210 or WIN 55,212–2 during measurement of synaptic transmission. Excitatory postsynaptic currents (EPSCs), evoked by electrical stimulation of afferent fibers, were significantly reduced in a dose-dependent manner by CB1 agonist application. EPSC inhibition was accompanied by an increase in two separate indices of presynaptic release, the paired-pulse response ratio and the coefficient of variation, suggesting a decrease in neurotransmitter release. These effects were prevented by application of the CB1 antagonist SR141716A. When Sr2+ was substituted for Ca2+ in the extracellular solution, application of HU-210 (1 μM) significantly reduced the frequency, but not amplitude, of evoked, asynchronous quantal release events. Spontaneous release events were similarly decreased in frequency with no change in amplitude. These findings further support the interpretation that CB1 activation leads to a decrease of glutamate release from afferent terminals in the striatum. These results reveal a novel potential role for cannabinoids in regulating striatal function and thus basal ganglia output and may suggest CB1-targeted drugs as potential therapeutic agents in the treatment of Parkinson's disease and other basal ganglia disorders. |
| Databáze: | OpenAIRE |
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