Mapping the expression of transient receptor potential channels across murine placental development

Autor: Joris Vriens, Katrien De Clercq, Vicente Perez-Garcia, Thomas Voets, Rieta Van Bree, Karen Peeraer, Federica Pollastro
Přispěvatelé: Vriens, Joris [0000-0002-2502-0409], Apollo - University of Cambridge Repository
Rok vydání: 2021
Předmět:
Placenta
Trophoblast stem cells
MOUSE
Mice
Transient receptor potential channel
Transient Receptor Potential Channels
0302 clinical medicine
Pregnancy
reproductive and urinary physiology
Calcium signaling
0303 health sciences
Chemistry
Stem Cells
Cell Differentiation
Mouse Embryonic Stem Cells
INTRAPLACENTAL YOLK-SAC
Trophoblasts
Cell biology
PREGNANCY
medicine.anatomical_structure
embryonic structures
GROWTH
Molecular Medicine
Original Article
Female
MATERNAL-FETAL CALCIUM
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
FUNCTIONAL EXPRESSION
TRPV2
TRPV Cation Channels
MICE LACKING
03 medical and health sciences
Cellular and Molecular Neuroscience
TRPM7
TRPM6
medicine
Animals
Calcium Signaling
Molecular Biology
Cell Proliferation
030304 developmental biology
Pharmacology
Science & Technology
TRP channels
Placental development
DELETION
Placentation
Trophoblast
Cell Biology
Mice
Inbred C57BL
Ion homeostasis
Gene Expression Regulation
CELLS
Calcium Channels
Primary trophoblast cells
030217 neurology & neurosurgery
Zdroj: CELLULAR AND MOLECULAR LIFE SCIENCES
r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
instname
r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
Centro de Investigación Principe Felipe (CIPF)
Cellular and Molecular Life Sciences
ISSN: 1420-9071
1420-682X
DOI: 10.1007/s00018-021-03837-3
Popis: Funder: Onderzoeksraad, KU Leuven; doi: http://dx.doi.org/10.13039/501100004497
Funder: Centre of Trophoblast Research, University of Cambridge
Transient receptor potential (TRP) channels play prominent roles in ion homeostasis by their ability to control cation influx. Mouse placentation is governed by the processes of trophoblast proliferation, invasion, differentiation, and fusion, all of which require calcium signaling. Although certain TRP channels have been shown to contribute to maternal–fetal transport of magnesium and calcium, a role for TRP channels in specific trophoblast functions has been disregarded. Using qRT-PCR and in situ hybridisation, the spatio-temporal expression pattern of TRP channels in the mouse placenta across gestation (E10.5–E18.5) was assessed. Prominent expression was observed for Trpv2, Trpm6, and Trpm7. Calcium microfluorimetry in primary trophoblast cells isolated at E14.5 of gestation further revealed the functional activity of TRPV2 and TRPM7. Finally, comparing TRP channels expression in mouse trophoblast stem cells (mTSCs) and mouse embryonic stem cells (mESC) confirmed the specific expression of TRPV2 during placental development. Moreover, TRP channel expression was similar in mTSCs compared to primary trophoblasts and validate mTSC as a model to study TRP channels in placental development. Collectivity, our results identify a specific spatio-temporal TRP channel expression pattern in trophoblasts, suggesting a possible involvement in regulating the process of placentation.
Databáze: OpenAIRE
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