Resistance pattern and assessment of phenicol agents' minimum inhibitory concentration in multiple drug resistant Chryseobacterium isolates from fish and aquatic habitats

Autor: Christian Michel, Brigitte Kerouault, Jf Bernardet, Oriane Matte-Tailliez
Přispěvatelé: Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2005
Předmět:
Florfenicol
MINIMUM INHIBITORY CONCENTRATION
medicine.drug_class
Antibiotics
Microbial Sensitivity Tests
Drug resistance
Chryseobacterium
Biology
Polymerase Chain Reaction
Applied Microbiology and Biotechnology
Agar dilution
Microbiology
03 medical and health sciences
Minimum inhibitory concentration
chemistry.chemical_compound
Drug Resistance
Multiple
Bacterial
medicine
Animals
Ecosystem
030304 developmental biology
FLORFENICOL
Thiamphenicol
0303 health sciences
030306 microbiology
Fishes
Gene Amplification
Dipeptides
General Medicine
Antimicrobial
biology.organism_classification
Anti-Bacterial Agents
3. Good health
CHRYSEOBACTERIUM
Blotting
Southern
Chloramphenicol
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
chemistry
Genes
Bacterial
MULTIPLE RESISTANCE
Efflux
EFFLUX MECHANISM
Water Microbiology
Biotechnology
ANTIBIOTIC
Zdroj: Journal of Applied Microbiology
Journal of Applied Microbiology, Wiley, 2005, 99 (2), pp.323-332. ⟨10.1111/j.1365-2672.2005.02592.x⟩
ISSN: 1364-5072
1365-2672
DOI: 10.1111/j.1365-2672.2005.02592.x⟩
Popis: Aims: To assess the susceptibility of Chryseobacterium isolates of fish and aquatic habitats to antimicrobial compounds. Special attention was paid to the resistance to chloramphenicol and florfenicol, a phenicol derivative recently licensed for use in veterinary medicine and fish farming. Methods and Results: Sixty-seven Chryseobacterium spp. isolates and reference strains, originating mainly from different aquatic habitats, were tested using the disk-diffusion method. In addition, agar dilution was used for assessing minimum inhibitory concentration of chloramphenicol and florfenicol. In spite of (i) conditions that hampered properly standardized experiments and (ii) the heterogeneity of the isolates resulting in some aberrant values in diffusion, correlation between the two methods was confirmed. Most of the isolates exhibited considerable multiresistance to most antimicrobial drug families, and many were clearly resistant to phenicols. Molecular investigations conducted on 10 strains selected for high resistance to florfenicol did not establish the existence of floR or cmlA genes currently reported in the literature as responsible for florfenicol resistance. Nevertheless, when an efflux pump inhibitor, phenyl-arginin-β-naphthylamide, was combined with diffusion tests, drug susceptibility to florfenicol was restored, suggesting that Chryseobacterium's resistance to this molecule is under the control of efflux mechanisms. Conclusions: Constitutive multiresistance to antibiotics is common in chryseobacteria isolated from the aquatic environment. Although no gene related to the floR family could be detected, efflux mechanisms could partly support the resistance to phenicols. Significance and Impact of the Study: These results explain the difficulty of treatment and clearly reflect the properties previously reported in Chryseobacterium isolates of human origin. Because several species have been involved in opportunistic infections in humans, the possible role of aquatic organisms as a source of infection should be considered.
Databáze: OpenAIRE
Externí odkaz: