Gastrin-releasing peptide receptor-based targeting using bombesin analogues is superior to metabolism-based targeting using choline for in vivo imaging of human prostate cancer xenografts
| Autor: | Chris H. Bangma, E. P. Krenning, Harald C. Groen, C. H. Grievink-de Ligt, Rogier P. J. Schroeder, Wouter A.P. Breeman, W.M. Van Weerden, E. de Blois, Saskia C. Berndsen, M. De Jong, Suzanne Reneman |
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| Přispěvatelé: | Urology, Radiology & Nuclear Medicine |
| Rok vydání: | 2011 |
| Předmět: |
Male
Fluorine Radioisotopes Positron emission tomography medicine.medical_specialty Xenograft model Gallium Radioisotopes Metabolism-based tracer Choline Mice chemistry.chemical_compound Prostate cancer SDG 3 - Good Health and Well-being Prostate Cell Line Tumor Internal medicine medicine Gastrin-releasing peptide receptor Animals Humans Radiology Nuclear Medicine and imaging Prostatic Neoplasms Bombesin Cancer General Medicine medicine.disease Receptors Bombesin Cell Transformation Neoplastic medicine.anatomical_structure Endocrinology chemistry Radiology Nuclear Medicine and imaging Positron-Emission Tomography Cancer cell Cancer research Original Article Oligopeptides Preclinical imaging |
| Zdroj: | European Journal of Nuclear Medicine and Molecular Imaging European Journal of Nuclear Medicine and Molecular Imaging, 38(7), 1257-1266. Springer-Verlag |
| ISSN: | 1619-7089 1619-7070 |
| Popis: | Purpose Prostate cancer (PC) is a major health problem. Overexpression of the gastrin-releasing peptide receptor (GRPR) in PC, but not in the hyperplastic prostate, provides a promising target for staging and monitoring of PC. Based on the assumption that cancer cells have increased metabolic activity, metabolism-based tracers are also being used for PC imaging. We compared GRPR-based targeting using the (68)Ga-labelled bombesin analogue AMBA with metabolism-based targeting using (18)F-methylcholine ((18)F-FCH) in nude mice bearing human prostate VCaP xenografts. Methods PET and biodistribution studies were performed with both (68)Ga-AMBA and (18)F-FCH in all VCaP tumour-bearing mice, with PC-3 tumour-bearing mice as reference. Scanning started immediately after injection. Dynamic PET scans were reconstructed and analysed quantitatively. Biodistribution of tracers and tissue uptake was expressed as percent of injected dose per gram tissue (%ID/g). Results All tumours were clearly visualized using (68)Ga-AMBA. (18)F-FCH showed significantly less contrast due to poor tumour-to-background ratios. Quantitative PET analyses showed fast tumour uptake and high retention for both tracers. VCaP tumour uptake values determined from PET at steady-state were 6.7 +/- 1.4%ID/g (20-30 min after injection, N=8) for (68)Ga-AMBA and 1.6 +/- 0.5%ID/g (10-20 min after injection, N=8) for (18)F-FCH, which were significantly different (p |
| Databáze: | OpenAIRE |
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