Screening of synthetic trehalose 6,6′-diesters and trehalose 6-monoesters as potential immunoreactants for the serodiagnosis of tuberculosis
| Autor: | M.B. Goren, V. Handzel, F Papa, H.H. Baer, Adalbert Laszlo |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Tuberculosis
Enzyme-Linked Immunosorbent Assay Microbiology Mycobacterium tuberculosis chemistry.chemical_compound Glycolipid Reference Values medicine Animals Humans Serologic Tests Molecular Biology Carbon chain Cord factor biology Trehalose General Medicine biology.organism_classification medicine.disease Biochemistry chemistry Polyclonal antibodies Immunoglobulin G biology.protein Rabbits Bacteria |
| Zdroj: | Research in Microbiology. 145:563-572 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/0923-2508(94)90033-7 |
| Popis: | The absence of serological cross-reactivity between trehalose 2,3-diester (DAT, formerly SL-IV) and synthetic trehalose 6,6′-diesters and trehalose 6-monoesters was established by ELISA testing using polyclonal immune sera raised in rabbits sensitized with “DAT”. From the screening of fifteen synthetic trehalose 6,6′- and 6-esters, “mirror” pseudo cord factor no. 1, “mirror” amides no. 5 and 6, cord factor analogues 7 and 8 and trehalose 6-monoesters 10 and 11 were selected for future, more extensive serological analysis. Paired comparisons of analogues among these fifteen substances showed that serodiagnostic discrimination power was more a function of the carbon chain length of their substituent groups — as well as of their position — than of the “mirror” constitution of the molecules. More exhaustive testing of these seven compounds is needed to select the synthetic product most efficient in the ELISA serodiagnosis of tuberculosis. |
| Databáze: | OpenAIRE |
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