INFLUENCE OF CARVEDILOL ON SUPEROXIDE GENERATION AND ENZYME RELEASE FROM STIMULATED HUMAN NEUTROPHILS
| Autor: | Dagmar Holománová, Radomír Nosál, Tatiana Mačičková, Jana Pečivová |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Neutrophils Carbazoles Stimulation In Vitro Techniques Antioxidants Neutrophil Activation General Biochemistry Genetics and Molecular Biology Propanolamines chemistry.chemical_compound Superoxides Internal medicine medicine Humans Receptor Carvedilol Antihypertensive Agents Peroxidase chemistry.chemical_classification Reactive oxygen species biology Superoxide Granule (cell biology) Enzyme Endocrinology chemistry Myeloperoxidase biology.protein medicine.drug |
| Zdroj: | Biomedical Papers. 149:389-392 |
| ISSN: | 1804-7521 1213-8118 |
| Popis: | Activation of neutrophils induces generation of reactive oxygen species and release of granule enzymes, which not only participate in the bactericidal mechanisms of these cells, but also in possible tissue damage. We studied the effect of carvedilol (CARV) [0.1-100 micromol/l], an antihypertensive and cardiovascular drug with antioxidative properties, on superoxide generation (SO) and myeloperoxidase (MPO) release from isolated human neutrophils stimulated with fMLP, a specific receptor activator, or with PMA, a receptor bypassing stimulus. Unstimulated cells showed neither SO formation nor MPO release after preincubation with drug. CARV decreased fMLP and PMA stimulated MPO release and SO generation dose dependently. The inhibitory effect of CARV may attributed to non-specific action since its effect was not influenced by the type of stimulation. It might inhibit SO generation as well as MPO release either by membrane-operating stimulus (fMLP) or membrane bypassing activator (PMA). |
| Databáze: | OpenAIRE |
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