D-alanylation of Teichoic Acids in Bacilli impedes the immune sensing of peptidoglycan in Drosophila
| Autor: | I. Gomberts Boneca, V. Sanchis Borja, Agnès Rejasse, Marie-Pierre Chapot-Chartier, L. El Chamy, Z. Attieh, Mireille Kallassy, Pascal Courtin |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Teichoic acid animal structures biology 030306 microbiology biology.organism_classification Cell biology Cell wall 03 medical and health sciences chemistry.chemical_compound Immune system chemistry Bacillus thuringiensis bacteria Peptidoglycan Lysozyme Cell wall modification Lactobacillus plantarum 030304 developmental biology |
| DOI: | 10.1101/631523 |
| Popis: | Modification of cell wall components is a prominent mean for pathogens to hinder host immune defenses. Here, using the Drosophila model, we aimed at characterizing the role of D-alanine esterification of teichoic acids (TAs) in the resistance of Bacillus thuringiensis to host defenses in vivo. We show that, by preventing cationic antimicrobial effectors-mediated bacterial lysis, this cell wall modification also limits the release of peptidoglycan immunostimulatory fragments thus impeding their sensing and the subsequent induction of the IMD- NF-κB pathway. Interestingly, we show that this strategy is also adopted by Lactobacillus plantarum, a bona fide commensal, to fine-tune its immunomodulatory potential in the Drosophila gut. Markedly, we show that the D-alanylation of TAs is essential for the resistance of L. plantarum to gut lysozyme. Altogether our data shed light on the mechanism underlying the persistence and the low immunostimulatory potential of L. plantarum in the Drosophila gut. |
| Databáze: | OpenAIRE |
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