Radioimmunoassay of somatostatin in human plasma
| Autor: | Harald Etzrodt, J Rosenthal, K. E. Schröder, Ernst-Friedrich Pfeiffer |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
endocrine system
medicine.medical_specialty Clinical Biochemistry Radioimmunoassay chemistry.chemical_element Peptide hormone Iodine Biochemistry Acetic acid chemistry.chemical_compound Antibody Specificity Internal medicine Blood plasma medicine Animals Humans Pancreas Antiserum Chromatography Histocytochemistry Immune Sera Biochemistry (medical) General Medicine Somatostatin Endocrinology chemistry Specific activity Rabbits hormones hormone substitutes and hormone antagonists |
| Zdroj: | Clinica Chimica Acta. 133:241-251 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(83)90268-1 |
| Popis: | A radioimmunoassay procedure for determination of somatostatin immunoactivity in human plasma is described. Labelling of tyrosine-1-somatostatin is performed by chloramine T. Specific activity of the tracer is 1.34 mol iodine for 1 mol of somatostatin. The immunoreactivity is 85%. Antisera are produced in rabbits at a titre of 179 × 10 −12 mol somatostatin per ml antiserum. Human plasma is extracted by n -butanol/2 mol/1 acetic acid. Recovery rate is 85–105%. The intra-assay CV varies from 12% to 1.7%, the inter-assay CV from 16% to 7.5%, depending on the concentration of somatostatin. |
| Databáze: | OpenAIRE |
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