30-bp deletion variant of LMP-1 gene of EBV in HIV+ patients After several years of antiretroviral therapy
| Autor: | Catherine Scieux, Janine Cherot, Félix Agbalika, Agnès Dumont |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty biology business.industry Virology law.invention chemistry.chemical_compound Infectious Diseases Real-time polymerase chain reaction chemistry Duplex (building) law Poster Presentation biology.protein medicine Antibody business Gene Viral load DNA Polymerase chain reaction Whole blood |
| Zdroj: | Retrovirology |
| ISSN: | 1742-4690 |
| Popis: | Materials and methods 19 cases well-documented were studied using the inhouse duplex polymerase chain reaction (PCR) with probes and primers overlapping 3'end of LMP1 on DNA extracts from whole blood. The results are given on the basis of Ct calculated using a pre-determined threshold for each run of deletion and non-deletion. Quantitative real time PCR was also performed in parallel on the same DNA extracts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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