The coactivator PGC-1α regulates skeletal muscle oxidative metabolism independently of the nuclear receptor PPARβ/δ in sedentary mice fed a regular chow diet
| Autor: | Christoph Handschin, Walter Wahli, Elyzabeth Vargas-Fernández, Joaquín Pérez-Schindler, Kristoffer Svensson, Gesa Santos |
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| Přispěvatelé: | Lee Kong Chian School of Medicine (LKCMedicine) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Transgene Blotting Western Peroxisome proliferator-activated receptor Muscle Skeletal/metabolism PPAR delta/genetics PPAR delta/metabolism PPAR-beta/genetics PPAR-beta/metabolism Physical Conditioning Animal/physiology Transcription Factors/genetics Transcription Factors/metabolism Context (language use) Oxidative phosphorylation Biology Article Mice Physical Conditioning Animal Internal medicine Internal Medicine medicine Animals PPAR delta Muscle Skeletal Receptor PPAR-beta chemistry.chemical_classification Skeletal muscle Calorimetry Indirect Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Science::Biological sciences [DRNTU] Endocrinology medicine.anatomical_structure Nuclear receptor chemistry Peroxisome proliferator-activated receptor delta Sedentary Behavior Transcription Factors |
| Zdroj: | Diabetologia Diabetologia, vol. 57, no. 11, pp. 2405-2412 |
| Popis: | Aims/hypothesis Physical activity improves oxidative capacity and exerts therapeutic beneficial effects, particularly in the context of metabolic diseases. The peroxisome proliferator-activated receptor (PPAR) γ coactivator-1α (PGC-1α) and the nuclear receptor PPARβ/δ have both been independently discovered to play a pivotal role in the regulation of oxidative metabolism in skeletal muscle, though their interdependence remains unclear. Hence, our aim was to determine the functional interaction between these two factors in mouse skeletal muscle in vivo. Methods Adult male control mice, PGC-1α muscle-specific transgenic (mTg) mice, PPARβ/δ muscle-specific knockout (mKO) mice and the combination PPARβ/δ mKO + PGC-1α mTg mice were studied under basal conditions and following PPARβ/δ agonist administration and acute exercise. Whole-body metabolism was assessed by indirect calorimetry and blood analysis, while magnetic resonance was used to measure body composition. Quantitative PCR and western blot were used to determine gene expression and intracellular signalling. The proportion of oxidative muscle fibre was determined by NADH staining. Results Agonist-induced PPARβ/δ activation was only disrupted by PPARβ/δ knockout. We also found that the disruption of the PGC-1α–PPARβ/δ axis did not affect whole-body metabolism under basal conditions. As expected, PGC-1α mTg mice exhibited higher exercise performance, peak oxygen consumption and lower blood lactate levels following exercise, though PPARβ/δ mKO + PGC-1α mTg mice showed a similar phenotype. Similarly, we found that PPARβ/δ was dispensable for PGC-1α-mediated enhancement of an oxidative phenotype in skeletal muscle. Conclusions/interpretation Collectively, these results indicate that PPARβ/δ is not an essential partner of PGC-1α in the control of skeletal muscle energy metabolism. Accepted version |
| Databáze: | OpenAIRE |
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