Liposomal formulations of oxybutynin and resiniferatoxin for the treatment of urinary diseases: improvement of drug tolerance upon intravesical
| Autor: | Daviane Maria Campos Vergne, Eliana B. Souto, Juliana Cordeiro Cardoso, Lorena M.D. Cirino, Patrícia Severino, Gabrielle Barrozo Novais, Francine Ferreira Padilha, Amanda Cano, Ricardo Luiz Cavalcanti de Albuquerque Júnior |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
medicine.medical_specialty
Urinalysis Urinary system Urology Resiniferatoxin Urinary incontinence Pharmaceutical Science Urine chemistry.chemical_compound Drug tolerance medicine Oxybutynin Liposome Science & Technology medicine.diagnostic_test business.industry Overactive bladder medicine.disease Inflammatory protection chemistry Liposomes business medicine.drug |
| Popis: | The use of liposomes for drug release has demonstrated to be a promising therapeutic platform for biomedical applications. In this study, intravesical administration of OXI (1.5 mM) and RTX (100 nM) was used to compare histological changes caused in Wistar female rats by the drugs both unloaded and loaded in liposomes. After instillation of formulations by intravesical catheter, bladders were removed and histological analysis carried out at pre-determined time intervals over a period of 60 days. Urinalysis was performed to verify the presence of infection and of liposomes. Results showed that RTX caused a higher bladder damage, with inflammatory reaction that reached all bladder layers. After 60 days, RTX-treated group showed urothelial alterations, collagen replacement by fibrosis and also abdominal adherence, but not the OXI-treated group. At the end of the assay, the liposomal-treated groups showed a minimal inflammatory reaction and significantly increased bladder size. Moreover, urinalysis confirmed the presence of liposomes in rat urine. RTX promoted higher bladder damage than OXI. Intravesical administration of liposomal OXI or RTX formulations minimized inflammatory reaction, with an extended drug effect on bladders. After a single intravesical administration, liposomes were found in rat urine samples after 60 days. This research was funded by Coordenacao Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Fundacao de Amparo a Pesquisa do Estado de Sergipe (FAPITEC), and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq). This work was also financed by the Portuguese Science and Technology Foundation, Ministry of Science and Education (FCT/MEC) for the project UIDB/04469/2020 (strategic fund), from national funds, and cofinanced by FEDER, under the Partnership Agreement PT2020. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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