Molecular characterization of HEPCIDIN-1 (HAMP1) gene in red-bellied pacu (Piaractus brachypomus)

Autor:	Julieth Michel Petano-Duque, Kelly Johanna Lozano-Villegas, Ángel Enrique Céspedes-Rubio, Iang Schroniltgen Rondón-Barragán
Rok vydání:	2022
Předmět:	Anti-Infective Agents Gene Expression Regulation Hepcidins Brain Injuries Immunology Animals RNA Messenger Adenosine Monophosphate Organophosphates Developmental Biology
Zdroj:	Developmental & Comparative Immunology. 130:104353
ISSN:	0145-305X
DOI:	10.1016/j.dci.2022.104353
Popis:	Hepcidins are cysteine-rich peptides, which participate in iron metabolism regulation, the inflammatory and antimicrobial response. This study characterizes the hepcidin-1 (HAMP1) gene, its transcript expression in different tissues, as well as its regulation in a model of brain injury in Piaractus brachypomus. Bioinformatic analysis was carried out to determine conserved domains, glycosylation sites and protein structure of HAMP1, and probability that HAMP1 corresponds to an antimicrobial peptide (AMP). Relative gene expression of the P. brachypomus HAMP1 gene was determined by qPCR from cDNA of several tissues, a brain injury model, an organophosphate sublethal toxicity model and anesthetic experiment using the 2
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::832642c959161fa95d13dcc68b255e99 https://doi.org/10.1016/j.dci.2022.104353 Zobrazit plný text záznamu Full Text from ScienceDirect